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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-GPCR GPR119 antibody
See all GPCR GPR119 products (3) ...
Description
Goat polyclonal to GPCR GPR119
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Rhesus monkey
Immunogen
Synthetic peptide: SARNCGPERPRESS, corresponding to C terminal amino acids 309-322 of Human GPCR GPR119 (NP_848566.1).
SARNCGPERP RESS
Positive control
Human Ovary lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab77391 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Western blot - GPCR GPR119 antibody (ab77391)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/2000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
Tissue specificity
Predominantly expressed in the pancreas, especially in the islets.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ8TDV5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- G protein coupled receptor 119 antibody
- G protein coupled receptor 2 antibody
- G-protein coupled receptor 119 antibody
see all
Database links
- Entrez Gene: 465858 Chimpanzee
- Entrez Gene: 139760 Human
- Omim: 300513 Human
- SwissProt: Q8TDV5 Human
- Unigene: 496762 Human
Anti-GPCR GPR119 antibody images:
Western blot - GPCR GPR119 antibody (ab77391)
Anti-GPCR GPR119 antibody (ab77391) at 0.3 µg/ml + Human Ovary lysate (in RIPA buffer) at 35 µg
Predicted band size : 37 kDa
Observed band size : 37 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-GPCR GPR119 antibody (ab77391)
ab77391 has not yet been referenced specifically in any publications.
Publishing research using ab77391? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"