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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-GPCR GPR125 antibody
See all GPCR GPR125 products (2) ...
Description
Rabbit polyclonal to GPCR GPR125
Tested applications
ICC/IF, ELISA, IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: TKRGNNTRTVDIVVLESSAQYCPPERV conjugated to KLH, corresponding to internal sequence amino acids 328-354 of Human GPCR GPR125
TKRGNNTRTV DIVVLESSAQ YCPPERV
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Immunocytochemistry/ Immunofluorescence - GPCR GPR125 antibody (ab51705)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab51705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-Fr
IHC-Fr: 1/100IHC-Fr: 1/100
IHC-P
IHC-P: 1/50IHC-P: 1/50
WB: Use at an assay dependent dilution.
Target
Relevance
GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
Cellular localization
Cell membrane; Multi pass membrane protein.
Alternative names
- FLJ38547 antibody
- G protein coupled receptor 125 antibody
- PGR21 antibody
see all
Database links
- Entrez Gene: 166647 Human
- Entrez Gene: 70693 Mouse
- Omim: 612303 Human
- SwissProt: Q8IWK6 Human
- SwissProt: Q7TT36 Mouse
Anti-GPCR GPR125 antibody images:
Immunocytochemistry/ Immunofluorescence - GPCR GPR125 antibody (ab51705)
ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GPCR GPR125 antibody (ab51705)
This product has been referenced in:
- He Zet al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P; Human.Read more (PubMed: 19846602) »
See 1 publication for this product
Publishing research using ab51705? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"