Anti-GPCR GPR48 antibody (ab75501)

Overview

  • Product nameAnti-GPCR GPR48 antibodySee all GPCR GPR48 primary antibodies ...
  • Description
    Rabbit polyclonal to GPCR GPR48
  • SpecificityDetects endogenous levels of total GPCR GPR48 protein.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of human GPCR GPR48

  • Positive control
    • Jurkat cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab75501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
ELISA 1/5000.

Target

  • FunctionOrphan receptor.
  • Tissue specificityExpressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession Q9BXB1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 48 antibody
    • G-protein coupled receptor 48 antibody
    • GPR48 antibody
    • Leucine-rich repeat-containing G protein-coupled receptor 4 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 4 antibody
    • LGR4 antibody
    • LGR4_HUMAN antibody
    see all

Anti-GPCR GPR48 antibody images

  • All lanes : Anti-GPCR GPR48 antibody (ab75501) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 104 kDa
    Observed band size : 104 kDa

References for Anti-GPCR GPR48 antibody (ab75501)

ab75501 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call. Please find the western blot protocol for use with ab75501 and ab75732 attached. For maximum signal, we recommend the overnight incubation at 4C with the primary antibody. Ab75501 should be used at a dilution bet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"