Anti-GPCR GPR81 antibody (ab106942)
Key features and details
- Goat polyclonal to GPCR GPR81
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPCR GPR81 antibody -
Description
Goat polyclonal to GPCR GPR81 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig -
Immunogen
Synthetic peptide:
C-QQLARQARMKKATR
, corresponding to internal sequence amino acids 207-220 of Human GPCR GPR81 (NP_115943.1). -
Positive control
- Human liver lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway. -
Tissue specificity
Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27198 Human
- Omim: 606923 Human
- SwissProt: Q9BXC0 Human
- Unigene: 610873 Human
- Unigene: 711001 Human
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Form
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. -
Alternative names
- FKSG80 antibody
- FKSG80 protein antibody
- G protein coupled receptor 104 antibody
see all
Images
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Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Datasheets and documents
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Datasheet download
References (2)
ab106942 has been referenced in 2 publications.
- Jia Q et al. Effects of GPR81 silencing combined with cisplatin stimulation on biological function in hypopharyngeal squamous cell carcinoma. Mol Med Rep 22:1727-1736 (2020). PubMed: 32582969
- Wagner W et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . PubMed: 26208712