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Western blot - GPR142 antibody (ab82147)
Western blot - GPR142 antibody (ab82147)
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Product Name 

GPR142 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to GPR142

Immunogen 

Synthetic peptide with sequence C-DMWRDTDSPRTLD, corresponding to internal sequence amino acids 298-310 of human GPR142 (NP_861455.1).
BLAST 'DMWRDTDSPRTLD' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 51 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver lysate.

Cellular localization 

Cell membrane; Multi pass membrane protein

Research areas 

Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR

Relevance 

GPR142 is a member of the rhodopsin family of G protein coupled receptors (GPRs). It is exclusively expressed in the central nervous system, most abundantly in the ventrolateral region of caudate putamen, the habenular nucleus, the zona incerta, and the medial mammillary nucleus.

 

GPR142 antibody (ab82147)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

350383    (Human)

Omim

    

609046    (Human)

SwissProt

    

Q7Z601    (Human)

Unigene

    

574368    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GPR142 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GPR142 antibody images, references, products related to ab82147 and other tools.

 

GPR142 antibody images:

Product image  Western blot - GPR142 antibody (ab82147)

Western blot - GPR142 antibody (ab82147)

GPR142 antibody (ab82147) at 1 µg/ml + human liver lysate in RIPA buffer at 35 µg

Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to GPR142

 

GPR142 antibody - more information

Customer reviews (feedback) regarding GPR142 antibody

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Price and availability of products related to GPR142 antibody

 

GPR142 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for GPR142 antibody

ab29879

  

Liver (Human) Membrane Lysate - adult normal tissue

 

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ab7004

  

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ab27245

  

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GPR142 antibody - other tools:

Contact Abcam with a Technical Enquiry about GPR142 antibody

 

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