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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Overview
Product name
Anti-GPR142 antibody
Description
Goat polyclonal to GPR142
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide with sequence C-DMWRDTDSPRTLD, corresponding to internal sequence amino acids 298-310 of human GPR142 (NP_861455.1).
DMWRDTDSPR TLD
Positive control
Human liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
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Western blot - GPR142 antibody (ab82147)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
GPR142 is a member of the rhodopsin family of G protein coupled receptors (GPRs). It is exclusively expressed in the central nervous system, most abundantly in the ventrolateral region of caudate putamen, the habenular nucleus, the zona incerta, and the medial mammillary nucleus.
Cellular localization
Cell membrane; Multi pass membrane protein
Alternative names
- G protein coupled receptor 142 antibody
- G protein coupled receptor PGR2 antibody
- PGR2 antibody
see all
Database links
- Entrez Gene: 350383 Human
- Omim: 609046 Human
- SwissProt: Q7Z601 Human
- Unigene: 574368 Human
Anti-GPR142 antibody images:
Western blot - GPR142 antibody (ab82147)
Anti-GPR142 antibody (ab82147) at 1 µg/ml + human liver lysate in RIPA buffer at 35 µg
Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-GPR142 antibody (ab82147)
ab82147 has not yet been referenced specifically in any publications.
Publishing research using ab82147? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"