Overview
- Product nameAnti-GPSM2 antibodySee all GPSM2 primary antibodies ...
- DescriptionRabbit polyclonal to GPSM2
- Tested applicationsICC/IF, IHC-Fr, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Zebrafish - Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (YFYLHDYAKA LEYHHHDLTL ARTIGDQLGE AKASGNLGNT LKVLGNFDEA) of Human GPSM2 (NP_037428).
- Positive controlHuman fetal liver lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab84571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
| IHC-P | IHC-P: Use a concentration of 5 µg/ml. |
Target
- FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
- Tissue specificityUbiquitously expressed.
- Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
- Sequence similaritiesBelongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats. - Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.
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Database links
- Entrez Gene: 29899 Human
- Entrez Gene: 29899 Human
- Entrez Gene: 76123 Mouse
- Entrez Gene: 362021 Rat
- Omim: 609245 Human
- SwissProt: P81274 Human
- SwissProt: P81274 Human
- SwissProt: Q8VDU0 Mouse
- Unigene: 584901 Human
- Unigene: 658489 Human
- Unigene: 226941 Mouse
- Unigene: 144235 Rat
see all
Target information above from: UniProt accession
P81274
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DFNB82 antibodyG protein signalling modulator 2 (AGS3 like C. elegans) antibodyG protein signalling modulator 2 antibody
- G-protein-signaling modulator 2 antibodyGpsm2 antibodyGPSM2_HUMAN antibodyHGNC:29501 antibodyLGN antibodyLGN protein antibodyMosaic protein LGN antibodyPins antibody
see all
Anti-GPSM2 antibody images
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Western blot - GPSM2 antibody (ab84571)Anti-GPSM2 antibody (ab84571) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 76 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - GPSM2 antibody (ab84571)ICC/IF image of ab84571 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPSM2 antibody (ab84571)ab84571 at 5 µg/ml staining GPSM2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPSM2 antibody (ab84571)ab84571 at 5 µg/ml staining GPSM2 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-GPSM2 antibody (ab84571)
ab84571 has not yet been referenced specifically in any publications.
