Overview

  • Product nameAnti-GRASP1 antibody
    See all GRASP1 primary antibodies
  • Description
    Rabbit polyclonal to GRASP1
  • Specificityab30576 recognises GRASP1.
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues from the C terminal region of rat GRASP1, conjugated to keyhole limpet hemocyanin (KLH).

  • Positive control
    • Rat cerebellar lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab30576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

Anti-GRASP1 antibody images

  • Anti-GRASP1 antibody (ab30576) at 1/1000 dilution + Rat cerebellar lysate

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
  • ICC/IF image of ab30576 stained PC12 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30576, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GRASP1 antibody (ab30576)

This product has been referenced in:
  • Tai HC  et al. Characterization of the Brain 26S Proteasome and its Interacting Proteins. Front Mol Neurosci 3: (2010). WB ; Rat . Read more (PubMed: 20717473) »

See 1 Publication for this product

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