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All lanes : Anti-GRB10 antibody (ab61771) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins) with no peptide
Lane 2 : extracts from NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins) with peptide
Predicted band size : 67 kDa
Observed band size : 30,40,67 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61771 at a dilution of 1/50. Left: sample untreated. Right: sample treated with immunising peptide.
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