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All lanes : Anti-GRB10 (phospho Y67) antibody (ab61795) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins)
Lane 2 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins) with immunising peptide
Predicted band size : 67 kDa
Observed band size : 67 kDa
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