|
|
|
|
Western blot - GRB 10 (phospho Y67) antibody (ab61795)
(enlarge)
|
|
|
|
|
Product Name
|
GRB10 (phospho Y67) antibody
|
| |
See all GRB10 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to GRB10 (phospho Y67)
|
|
Immunogen
|
Synthetic phosphopeptide derived from human GRB 10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
ab61795 detects endogenous levels of GRB 10 only when phosphorylated at tyrosine 67.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins).
|
|
Cellular localization
|
Cytoplasmic, Plasma membrane
|
|
Research areas
|
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic
|
|
Relevance
|
GRB 10 belongs to a small family of adapter proteins that interact with receptor tyrosine kinases and signaling molecules. In particular, GRB 10 interacts with insulin receptors and insulin-like growth-factor receptors. Alternatively spliced transcript variants encoding different isoforms have been identified: overexpression of some of them inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
ab61795 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GRB10 (phospho Y67) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for GRB10 (phospho Y67) antibody images, references, products related to ab61795 and other tools.
|
| |
GRB10 (phospho Y67) antibody images:
|
 Western blot - GRB 10 (phospho Y67) antibody (ab61795)
All lanes : GRB10 (phospho Y67) antibody (ab61795) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins)
Lane 2 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins) with immunising peptide
Predicted band size : 67 kDa
Observed band size : 67 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to GRB10
|
| |
GRB10 (phospho Y67) antibody - more information
|
Customer reviews (feedback) regarding GRB10 (phospho Y67) antibody |
Customer FAQs regarding GRB10 (phospho Y67) antibody |
Protocols for GRB10 (phospho Y67) antibody |
Price and availability of products related to GRB10 (phospho Y67) antibody |
| |
GRB10 (phospho Y67) antibody - related products:
|
|
|
| |
GRB10 (phospho Y67) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about GRB10 (phospho Y67) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
