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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-GRB10 (phospho Y67) antibody
See all GRB10 products (4) ...
Description
Rabbit polyclonal to GRB10 (phospho Y67)
Specificity
ab61795 detects endogenous levels of GRB 10 only when phosphorylated at tyrosine 67.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide derived from human GRB 10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).
Positive control
NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61795 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic
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Western blot - GRB 10 (phospho Y67) antibody (ab61795)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Target
Function
Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
Tissue specificity
Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
Sequence similarities
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
Domain
The PH domain binds relatively non-specifically to several phosphoinositides, including PI(5)P, PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3, with modest affinities.
Post-translational
modifications
phosphorylated on serine residues upon EGF, FGF and PDGF stimulation.
Cellular localization
Cytoplasm. When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment.
Target information above from: UniProt accessionQ13322
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- grb 10 antibody
- GRB IR antibody
- grb-10 antibody
see all
Database links
- Entrez Gene: 2887 Human
- Omim: 601523 Human
- SwissProt: Q13322 Human
- Unigene: 164060 Human
Anti-GRB10 (phospho Y67) antibody images:
Western blot - GRB 10 (phospho Y67) antibody (ab61795)
All lanes : Anti-GRB10 (phospho Y67) antibody (ab61795) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins)
Lane 2 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins) with immunising peptide
Predicted band size : 67 kDa
Observed band size : 67 kDa
References for Anti-GRB10 (phospho Y67) antibody (ab61795)
ab61795 has not yet been referenced specifically in any publications.
Publishing research using ab61795? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"