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Western blot - GRB 10 (phospho Y67) antibody (ab61795)
Western blot - GRB 10 (phospho Y67) antibody (ab61795)
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Product Name 

GRB10 (phospho Y67) antibody

 

See all GRB10 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GRB10 (phospho Y67)

Immunogen 

Synthetic phosphopeptide derived from human GRB 10 around the phosphorylation site of tyrosine 67 (S-L-YP-S-A).

Reacts with 
(species key)

Hu

Specificity 

ab61795 detects endogenous levels of GRB 10 only when phosphorylated at tyrosine 67.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins).

Cellular localization 

Cytoplasmic, Plasma membrane

Research areas 

Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic

Relevance 

GRB 10 belongs to a small family of adapter proteins that interact with receptor tyrosine kinases and signaling molecules. In particular, GRB 10 interacts with insulin receptors and insulin-like growth-factor receptors. Alternatively spliced transcript variants encoding different isoforms have been identified: overexpression of some of them inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner.

 

Alternative names GRB10 (phospho Y67) antibody (ab61795)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2887    (Human)

Omim

    

601523    (Human)

SwissProt

    

Q13322    (Human)

Unigene

    

164060    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab61795 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GRB10 (phospho Y67) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GRB10 (phospho Y67) antibody images, references, products related to ab61795 and other tools.

 

GRB10 (phospho Y67) antibody images:

Product image  Western blot - GRB 10 (phospho Y67) antibody (ab61795)

Western blot - GRB 10 (phospho Y67) antibody (ab61795)

All lanes : GRB10 (phospho Y67) antibody (ab61795) at 1/500 dilution

Lane 1 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins)
Lane 2 : NIH 3T3 cell extracts treated with Insulin (0.01U/ml, 15mins) with immunising peptide


Predicted band size : 67 kDa
Observed band size : 67 kDa

 
 

Search PubMed (MEDLINE) for references to GRB10

 

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GRB10 (phospho Y67) antibody - related products:

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Loading controls

Lysates

 

Positive Controls for GRB10 (phospho Y67) antibody

ab14873

  

NIH 3T3 (Mouse embryonic fibroblast cell line) Cytoplasmic lysate

 

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Other GRB10 Antibodies

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GRB10 (phospho Y67) antibody - other tools:

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