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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-GRB2 antibody [GRB-232]
See all GRB2 products (15) ...
Description
Mouse monoclonal [GRB-232] to GRB2
Specificity
Reacts specifically with an epitope in the C-terminal region of Grb2 (a.a. 200-217).
Tested applications
IP, WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus Monkey
Immunogen
Synthetic peptide: GQTGMFPRNYVTPVNRNV conjugated to KLH, corresponding to C terminal amino acids 200-217 of Human GRB2
GQTGMFPRNY VTPVNRNV
Positive control
Whole extract of cultured human acute T cell leukemia Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purified from culture supernatant of hybridoma cells.
Clonality
Monoclonal
Clone number
GRB-232
Isotype
IgG3
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 25 kDa.
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localization
Golgi apparatus.
Target information above from: UniProt accessionP62993
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Database links
- Entrez Gene: 386572 Chicken
- Entrez Gene: 535298 Cow
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Entrez Gene: 406308 Zebrafish
- Omim: 108355 Human
- SwissProt: Q07883 Chicken
see all
References for Anti-GRB2 antibody [GRB-232] (ab49876)
This product has been referenced in:
- Fritsche Let al. Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907. Am J Physiol Endocrinol Metab 300:E824-36 (2011).Read more (PubMed: 21098738) »
- Chentouf Met al. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets. PLoS One 6:e22954 (2011). WB; Rat.Read more (PubMed: 21826222) »
See all 2 publications for this product
Publishing research using ab49876? Please let us know so that we can cite the reference in this datasheet
![GRB2 antibody [GRB-232] for WB in Human (49876)](/ps/Reviews/Images/ab9387_46501.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"