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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32037? Please let us know so that we can cite the reference in this datasheet
ab32037 has been referenced in 1 publications.
- Moses J et al. Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting. RNA 16:430-41 (2010). WB; Human. PubMed: 20026621
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037WB.jpg)
Western blot - GRB2 antibody [Y237] (ab32037)
Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution + 293 cell lysate
Predicted band size : 25 kDa
Observed band size : 25 kDa
![Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037IHCpara.jpg)
Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237] (ab32037)
Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
![Immunofluorescence - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037IFA.jpg)
Immunofluorescence - GRB2 antibody [Y237] (ab32037)
Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037.
![Western blot - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/images/32/ab32037/GRB2-Primary-antibodies-ab32037-3.jpg)
Western blot - GRB2 antibody [Y237] (ab32037)
Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is a courtesy of Anonymous Abreview
![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](/ps/datasheet/images/32/ab32037/GRB2-Primary-antibodies-ab32037-5.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)
Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
![GRB2 antibody [Y237] for WB in Human (32037)](/ps/Reviews/Images/ab9271_45595.jpg)
GRB2 antibody [Y237] for WB in Human (32037)
![GRB2 antibody [Y237] for WB in Human (32037)](/ps/datasheet/images/32/ab32037/GRB2-Primary-antibodies-ab32037-2.gif)
GRB2 antibody [Y237] for WB in Human (32037)
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