![Western blot - GRB2 antibody [Y237] (ab32037) image](#ab32037WB.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237] (ab32037) image](#ab32037IHCpara.jpg)
![Immunofluorescence - GRB2 antibody [Y237] (ab32037) image](#ab32037IFA.jpg)
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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-GRB2 antibody [Y237]
See all GRB2 products (15) ...
Description
Rabbit monoclonal [Y237] to GRB2
Specificity
This antibody can also detect the splice isoform GRB3-3 of GRB2.
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the C terminus of human GRB2
Positive control
293 cell lysate, human ovarian carcinoma, HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
Y237
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/50 - 1/100.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localization
Golgi apparatus.
Target information above from: UniProt accessionP62993
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Database links
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
- SwissProt: P62994 Rat
- Unigene: 444356 Human
see all
Anti-GRB2 antibody [Y237] images:
Western blot - GRB2 antibody [Y237] (ab32037)
![Western blot - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037WB.jpg)
Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution + 293 cell lysate
Predicted band size : 25 kDa
Observed band size : 25 kDa
Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237] (ab32037)
![Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037IHCpara.jpg)
Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
Immunofluorescence - GRB2 antibody [Y237] (ab32037)
![Immunofluorescence - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/Images/32/ab32037/ab32037IFA.jpg)
Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037.
Western blot - GRB2 antibody [Y237] (ab32037)
![Western blot - GRB2 antibody [Y237] (ab32037)](/ps/datasheet/images/32/ab32037/GRB2-Primary-antibodies-ab32037-3.jpg)
Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is a courtesy of Anonymous Abreview
References for Anti-GRB2 antibody [Y237] (ab32037)
This product has been referenced in:
- Moses Jet al. Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting. RNA 16:430-41 (2010). WB; Human.Read more (PubMed: 20026621) »
See 1 publication for this product
Publishing research using ab32037? Please let us know so that we can cite the reference in this datasheet
![GRB2 antibody [Y237] for WB in Human (32037)](/ps/Reviews/Images/ab9271_45595.jpg)
![GRB2 antibody [Y237] for WB in Human (32037)](/ps/datasheet/images/32/ab32037/GRB2-Primary-antibodies-ab32037-2.gif)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"