GRB2 antibody [Y237] (ab32037)

Product Name 

GRB2 antibody [Y237]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y237] to GRB2

Immunogen 

Synthetic peptide corresponding to residues in the C terminus of human GRB2

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody can also detect the splice isoform GRB3-3 of GRB2.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/50 - 1/100.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

293 cell lysate, human ovarian carcinoma, HeLa cells.

Cellular localization 

Golgi Apparatus

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic

Relevance 

Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y237

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for GRB2 antibody [Y237] images, references, products related to ab32037 and other tools.

GRB2 antibody [Y237] images:

References for GRB2 antibody [Y237] (ab32037)

  This product has been used in:

Moses J et al. Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting. RNA 16:430-41 (2010). WB; Human. PubMed: 20026621

If you publish research using ab32037 please let us know so that we can cite the reference on this datasheet.

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