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Anti-GRB2 antibody [Y301] (ab32111)

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2 questions for ab32111

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Question 1

Monday 23-January-2012

What is the recommended antigen retrieval buffer for these three antibodies?

ANSWER:

  

Thank you for contacting us.

The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but a steamer or waterbath at 95-100C will be an acceptable substitute.

Place slides in a container of 0.01 M sodium citrate buffer, pH 6.0 heated to 100°C and maintain this temperature for 20 minutes by placing the container in a steamer or heated waterbath. After 20 minutes, remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Question 2

Monday 24-October-2011

What was the antigen retrieval method that was used to generate the picture on your datasheet?

ANSWER:

  

We used a heat mediated antigen retrieval method with sodium citrate, pH. 6.0.

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