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Anti-GRB2 antibody [Y301] (ab32111)

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Overview

Product name

Anti-GRB2 antibody [Y301]
See all GRB2 products (15) ...

Description

Rabbit monoclonal [Y301] to GRB2

Specificity

ab32111 recognises GRB2. This antibody can also detect the splice isoform GRB3-3 of GRB2.

Tested applications

WB, IHC-P, ICC/IF, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the N terminus of human GRB2

Positive control

Human breast carcinoma, NIH3T3 cells, PC12 cells, HeLa cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y301

Isotype

IgG

Research areas

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic

  • Western blot - GRB2 antibody [Y301] (ab32111)Western blot - GRB2 antibody [Y301] (ab32111) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y301] (ab32111)Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y301] (ab32111) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - GRB2 antibody [Y301] (ab32111)Immunocytochemistry/ Immunofluorescence - GRB2 antibody [Y301] (ab32111) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or IP.

Target

Function

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Sequence similarities

Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.

Domain

The SH3 domains mediate interaction with RALGPS1 and SHB.

Cellular localization

Golgi apparatus.

Target information above from: UniProt accessionP62993 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Abundant SRC homology antibody
  • Adapter protein GRB2 antibody
  • ASH antibody
  • Ash protein antibody
  • EGFRBP GRB2 antibody
  • Epidermal growth factor receptor binding protein antibody
  • Epidermal growth factor receptor binding protein GRB2 antibody
  • GRB 2 antibody
  • GRB2 adapter protein antibody
  • Grb2 antibody
  • GRB2_HUMAN antibody
  • Grb3 3 antibody
  • Growth Factor Receptor Bound Protein 2 antibody
  • Growth Factor Receptor Bound Protein 2 antibody
  • Growth factor receptor bound protein 3 antibody
  • Growth factor receptor-bound protein 2 antibody
  • HT027 antibody
  • MST084 antibody
  • MSTP084 antibody
  • OTTHUMP00000166096 antibody
  • OTTHUMP00000166097 antibody
  • OTTHUMP00000166098 antibody
  • Protein ASH antibody
  • SEM5 antibody
  • SEM5 antibody
  • SH2/SH3 adapter GRB2 antibody
see all

Database links

see all

Anti-GRB2 antibody [Y301] images:

  Western blot - GRB2 antibody [Y301] (ab32111)

Western blot - GRB2 antibody [Y301] (ab32111)

All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/2000 dilution

Lane 1 : NIH 3T3 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : HeLa cell lysate


Predicted band size : 25 kDa
Observed band size : 25 kDa

  Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y301] (ab32111)

Immunohistochemistry (Paraffin-embedded sections) - GRB2 antibody [Y301] (ab32111)

Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111.

  Immunocytochemistry/ Immunofluorescence - GRB2 antibody [Y301] (ab32111)

Immunocytochemistry/ Immunofluorescence - GRB2 antibody [Y301] (ab32111)

Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.

References for Anti-GRB2 antibody [Y301] (ab32111)

ab32111 has not yet been referenced specifically in any publications.

Publishing research using ab32111? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"