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Anti-GRB2 antibody (ab2234)

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Overview

Product name

Anti-GRB2 antibody
See all GRB2 products (15) ...

Description

Goat polyclonal to GRB2

Tested applications

ELISA, IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide: PRNYVTPVNRNV, corresponding to C terminal amino acids 206-217 of Human GRB2.

C-PRNYVTPV NRNV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic

  • Western blot - GRB2 antibody (ab2234)Western blot - GRB2 antibody (ab2234) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRB2 antibody (ab2234)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRB2 antibody (ab2234) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 0.1 - 0.3 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Sequence similarities

Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.

Domain

The SH3 domains mediate interaction with RALGPS1 and SHB.

Cellular localization

Golgi apparatus.

Target information above from: UniProt accessionP62993 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Abundant SRC homology antibody
  • Adapter protein GRB2 antibody
  • ASH antibody
  • Ash protein antibody
  • EGFRBP GRB2 antibody
  • Epidermal growth factor receptor binding protein antibody
  • Epidermal growth factor receptor binding protein GRB2 antibody
  • GRB 2 antibody
  • GRB2 adapter protein antibody
  • Grb2 antibody
  • GRB2_HUMAN antibody
  • Grb3 3 antibody
  • Growth Factor Receptor Bound Protein 2 antibody
  • Growth factor receptor bound protein 3 antibody
  • Growth factor receptor-bound protein 2 antibody
  • HT027 antibody
  • MST084 antibody
  • MSTP084 antibody
  • OTTHUMP00000166096 antibody
  • OTTHUMP00000166097 antibody
  • OTTHUMP00000166098 antibody
  • Protein ASH antibody
  • SEM5 antibody
  • SH2/SH3 adapter GRB2 antibody
see all

Database links

see all

Anti-GRB2 antibody images:

  Western blot - GRB2 antibody (ab2234)

Western blot - GRB2 antibody (ab2234)

Anti-GRB2 antibody (ab2234) at 2 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane).

Predicted band size : 30 kDa
Observed band size : 30 kDa


Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRB2 antibody (ab2234)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRB2 antibody (ab2234)

Ab2234 (0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

References for Anti-GRB2 antibody (ab2234)

ab2234 has not yet been referenced specifically in any publications.

Publishing research using ab2234? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"