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Product name
GRB2 protein (His tag)
See all GRB2 products (6) ...
Protein description
Recombinant full length protein: Human GRB2 protein fused with His-tag (N-terminus).
Expression host
E. coli
Properties
Purity
> 95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 2.5mM Imidazole, 3mM Sodium chloride, 10mM Tris, pH 8.0
Concentration
Concentration information loading...
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Adapters >> Cytoplasmic
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SDS-PAGE - GRB2 protein (ab40619)
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Applications
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Our Abpromise guarantee covers the use of ab40619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
MS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localization
Golgi apparatus.
Target information above from: UniProt accessionP62993
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
GRB2 protein (His tag) images:
SDS-PAGE - GRB2 protein (ab40619)
SDS PAGE analysis of GRB2 Recombinant protein (1µg) stained with Coomassie blue.
References for GRB2 protein (His tag) (ab40619)
ab40619 has not yet been referenced specifically in any publications.
Publishing research using ab40619? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"