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Western blot - GREB1 antibody (ab84025)
Western blot - GREB1 antibody (ab84025)
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Product Name 

GREB1 antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to GREB1

Immunogen 

Synthetic peptide within residues: DNGHNALLGF SGNCVGCGKK GFCYFTEFSN HINLKLTTQP KKQKHLKYYL , corresponding to internal sequence amino acids 144-193 of Human GREB1(NP_683701)
BLAST 'DNGHNALLGF SGNCVGCGKK GFCYFTEFSN HINLKLTTQP KKQKHLKYYL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 cell lysate.

Cellular localization 

Single-pass membrane protein

Research areas 

Cancer >> Growth factors >> Hormones

Relevance 

GREB1 is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to act as a regulator of hormone-dependent cancer growth in breast and prostate cancers. Its promoter contains putative androgen receptor response elements. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene

 

Alternative names GREB1 antibody (ab84025)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9687    (Human)
268527    (Mouse)

Omim

    

611736    (Human)

SwissProt

    

Q4ZG55    (Human)
Q3UHK3    (Mouse)

Unigene

    

467733    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

ab84025 is purified by a peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GREB1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GREB1 antibody images, references, products related to ab84025 and other tools.

 

GREB1 antibody images:

Product image  Western blot - GREB1 antibody (ab84025)

Western blot - GREB1 antibody (ab84025)

GREB1 antibody (ab84025) at 1 µg/ml + MCF7 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 43 kDa
Observed band size : 43 kDa

 
 

Search PubMed (MEDLINE) for references to GREB1

 

GREB1 antibody - more information

Customer reviews (feedback) regarding GREB1 antibody

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Price and availability of products related to GREB1 antibody

 

GREB1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for GREB1 antibody

ab29539

  

MCF7 (Human breast adenocarcinoma cell line) Membrane Lysate - tumor cell line

 

Compatible Secondaries for GREB1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other GREB1 Antibodies

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GREB1 antibody - other tools:

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