Anti-GRIK5 antibody (ab85441)

Overview

  • Product nameAnti-GRIK5 antibody
    See all GRIK5 primary antibodies
  • Description
    Rabbit polyclonal to GRIK5
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 of Human GRIK5 (

    EDGLYGAPEPNGSWTGMVGELINRKADLAVAAFTITAEREKVIDFSKPFM

    )

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAA 2 antibody
    • EAA2 antibody
    • Excitatory amino acid receptor 2 antibody
    • GluK5 antibody
    • GluRgamma2 antibody
    • Glutamate receptor antibody
    • Glutamate receptor KA 2 antibody
    • Glutamate receptor KA-2 antibody
    • Glutamate receptor KA2 antibody
    • Glutamate receptor, ionotropic kainate 5 [Precursor] antibody
    • Glutamate receptor, ionotropic, kainate 5 (gamma 2) antibody
    • Glutamate receptor, ionotropic, kainate 5 antibody
    • GRIK 2 antibody
    • GRIK 5 antibody
    • GRIK2 antibody
    • Grik5 antibody
    • GRIK5_HUMAN antibody
    • iGlu5 antibody
    • ionotropic kainate 5 antibody
    • KA2 antibody
    • MGC118086 antibody
    see all

Anti-GRIK5 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling GRIK5 with ab85441 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the membrane of cell processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - GRIK5. Right - Merge.
  • Anti-GRIK5 antibody (ab85441) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 109 kDa
  • Immunohistochemistry of Mouse Retina tissue at an antibody concentration of 25µg/ml using anti-GRIK5 antibody (ab85441)

References for Anti-GRIK5 antibody (ab85441)

ab85441 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Monkey Cultured Cells (COS-7)
Specification COS-7
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Taro Fujikawa

Verified customer

Submitted Mar 30 2012

I am very pleased to hear you would like to accept our offer and test ab85441 in ICC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the Addit...

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Thank you very much for your interest in ab85441 and ab118954.

To our knowledge, ab85441 and ab118954 have not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buy ab85441 and/or 118954now, test it inICC and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"