Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-GRIM19 antibody
See all GRIM19 products (7) ...
Description
Mouse monoclonal to GRIM19
Tested applications
WB, ICC/IF, ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Recombinant full length protein Human GRIM19.
Positive control
Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria, Human fibroblasts, HeLa cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Epigenetics and Nuclear Signaling >> Cell cycle >> Apoptosis >> Nuclear
Cell Biology >> Apoptosis >> Nucleus >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF: Use a concentration of 1 µg/ml.
ELISA: Use a concentration of 8 µg/ml. In-Cell ELISA (0.8 µg/well)
Flow Cyt: Use a concentration of 1 µg/ml.
Target
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
Tissue specificity
Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
Involvement in disease
Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
Sequence similarities
Belongs to the complex I NDUFA13 subunit family.
Developmental stage
Expressed in numerous fetal tissues.
Cellular localization
Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment.
Target information above from: UniProt accessionQ9P0J0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2700054G14Rik antibody
- AU022060 antibody
- B16.6 antibody
see all
Database links
- Entrez Gene: 338084 Cow
- Entrez Gene: 51079 Human
- Entrez Gene: 67184 Mouse
- Entrez Gene: 314759 Rat
- Omim: 609435 Human
- SwissProt: Q95KV7 Cow
- SwissProt: Q9P0J0 Human
- SwissProt: Q9ERS2 Mouse
see all
Anti-GRIM19 antibody images:
Western blot - Anti-GRIM19 antibody (ab110240)
All lanes : Anti-GRIM19 antibody (ab110240) at 1 µg/ml
Lane 1 : Human heart mitochondria
Lane 2 : Bovine heart mitochondria
Lane 3 : Rat heart mitochondria
Lane 4 : Mouse heart mitochondria
Predicted band size : 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody (ab110240)
Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
Flow Cytometry - Anti-GRIM19 antibody (ab110240)
HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
References for Anti-GRIM19 antibody (ab110240)
This product has been referenced in:
- Lebon S et al. A novel mutation of the NDUFS7 gene leads to activation of a cryptic exon and impaired assembly of mitochondrial complex I in a patient with Leigh syndrome. Mol Genet Metab 92:104-8 (2007).Read more (PubMed: 17604671) »
- Li Y et al. An assembled complex IV maintains the stability and activity of complex I in mammalian mitochondria. J Biol Chem 282:17557-62 (2007).Read more (PubMed: 17452320) »
See all 12 publications for this product
Publishing research using ab110240? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"