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Overview

  • Product nameGRO alpha Mouse ELISA KitSee all GRO alpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    0.614 pg/ml - 150 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 123.5 115% - 139%
    Serum 89.3 78% - 97%
    Plasma 101.4 88% - 113%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100717 mouse GRO alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse GRO alpha in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse GRO alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and GRO alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GRO alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of KC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • RelevanceThe GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93% and 82% identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.
    • Alternative names
        C-X-C motif chemokine 1chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)fibroblast secretory protein
        FSPGROGRO1GRO1 oncogene (melanoma growth stimulating activity, alpha)GRO1 oncogene (melanoma growth-stimulating activity)GROagrowth-regulated alpha proteinMelanoma growth stimulatory activitymelanoma growth stimulatory activity alphaMGSAMGSA aMGSA alphaNAP 3Neutrophil-activating protein 3SCYB1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100717 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      GRO alpha Mouse ELISA Kit images

      • ELISA - GRO alpha Mouse ELISA Kit (ab100717)
        ELISA - GRO alpha Mouse ELISA Kit (ab100717)

      Protocols

      References for GRO alpha Mouse ELISA Kit (ab100717)

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