|
|
|
|
|
Product Name
|
GRP78 BiP antibody [76-E6]
|
| |
See all GRP78 BiP - ER marker antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rat monoclonal [76-E6] to GRP78 BiP
|
|
Immunogen
|
The details of the immunogen for this antibody are not available.
|
|
Reacts with
(species key)
|
Ms, Hm
|
|
Specificity
|
This antibody has been shown to be useful in studies of immunoglobulin assembly and secretion.
|
|
Epitope
|
This antibody recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
Studies of immunoglobulin assembly and secretion: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Endoplasmic reticulum lumen.
|
|
Research areas
|
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
|
|
Relevance
|
The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 14828 (Mouse)
|
| |
|
|
Raised in
|
Rat
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
76-E6
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
100 mM borate buffered saline, pH 8.0.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GRP78 BiP antibody [76-E6] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to GRP78 BiP
|
| |
GRP78 BiP antibody [76-E6] - more information
|
Customer reviews (feedback) regarding GRP78 BiP antibody [76-E6] |
Customer FAQs regarding GRP78 BiP antibody [76-E6] |
Protocols for GRP78 BiP antibody [76-E6] |
Price and availability of products related to GRP78 BiP antibody [76-E6] |
| |
GRP78 BiP antibody [76-E6] - related products:
|
|
|
| |
GRP78 BiP antibody [76-E6] - other tools:
|
Contact Abcam with a Technical Enquiry about GRP78 BiP antibody [76-E6] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
