Publishing research using ab21685? Please let us know so that we can cite the reference in this datasheet
ab21685 has been referenced in 23 publications.
- Auricchio N et al. Therapeutic trial of metformin and bortezomib in a mouse model of tuberous sclerosis complex (TSC). PLoS One 7:e31900 (2012).
IHC-P;
Mouse.
PubMed: 22363765
- Randazzo J et al. Orally active multi-functional antioxidants delay cataract formation in streptozotocin (type 1) diabetic and gamma-irradiated rats. PLoS One 6:e18980 (2011).
WB;
Rat.
PubMed: 21541328
- Das S & Pellett PE Spatial Relationships between Markers for Secretory and Endosomal Machinery in Human Cytomegalovirus-Infected Cells versus Those in Uninfected Cells. J Virol 85:5864-79 (2011).
PubMed: 21471245
- Johnson JS et al. AAV Exploits Subcellular Stress Associated with Inflammation, Endoplasmic Reticulum Expansion, and Misfolded Proteins in Models of Cystic Fibrosis. PLoS Pathog 7:e1002053 (2011).
PubMed: 21625534
- Milenkovic VM et al. Disease-associated missense mutations in bestrophin-1 affect cellular trafficking and anion conductance. J Cell Sci 124:2988-96 (2011).
ICC/IF;
Dog.
PubMed: 21878505
- Bewley MA et al. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae. Mol Cell Proteomics 10:M111.008193 (2011).
WB;
Human.
PubMed: 21474794
- Chai S et al. Activation of acid-sensing ion channel 1a (ASIC1a) by surface trafficking. J Biol Chem 285:13002-11 (2010).
ICC/IF;
Chinese Hamster.
PubMed: 20185828
- Jerome-Majewska LA et al. The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta. Dev Biol 341:154-66 (2010).
PubMed: 20178780
- Dykstra KM et al. Yip1A structures the mammalian endoplasmic reticulum. Mol Biol Cell 21:1556-68 (2010).
PubMed: 20237155
- Drori A et al. Prolonged endoplasmic reticulum stress promotes mislocalization of immunoglobulins to the cytoplasm. Mol Immunol 47:1719-27 (2010).
PubMed: 20353876
- Bartoi T et al. GABAB receptor constituents revealed by tandem affinity purification from transgenic mice. J Biol Chem 285:20625-33 (2010).
WB;
Mouse.
PubMed: 20406808
- Ravikumar B et al. Plasma membrane contributes to the formation of pre-autophagosomal structures. Nat Cell Biol 12:747-57 (2010).
ICC/IF;
Human.
PubMed: 20639872
- Song J et al. Severe burn-induced endoplasmic reticulum stress and hepatic damage in mice. Mol Med 15:316-20 (2009).
WB;
Mouse.
PubMed: 19603103
- Zwang NA et al. Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry. J Proteome Res 8:1540-54 (2009).
IHC-P, Electron Microscopy;
Rat.
PubMed: 19209902
- Pasquariello N et al. Characterization of the Endocannabinoid System in Human Neuronal Cells and Proteomic Analysis of Anandamide-induced Apoptosis. J Biol Chem 284:29413-26 (2009).
WB;
Human.
PubMed: 19690173
- Smith L et al. Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome? Neoplasia 11:1194-207 (2009).
WB;
Human.
PubMed: 19881955
- Sørensen BS et al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother Oncol 92:443-9 (2009).
WB;
Human.
PubMed: 19541378
- Nielsen J et al. Proteomic analysis of lithium-induced nephrogenic diabetes insipidus: mechanisms for aquaporin 2 down-regulation and cellular proliferation. Proc Natl Acad Sci U S A 105:3634-9 (2008).
WB;
Rat.
PubMed: 18296634
- Parkyn CJ et al. LRP1 controls biosynthetic and endocytic trafficking of neuronal prion protein. J Cell Sci 121:773-83 (2008).
ICC/IF;
Mouse.
PubMed: 18285446
- Nunes P et al. A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2 (AQP2). Am J Physiol Cell Physiol : (2008).
PubMed: 18799651
- Verras M et al. Tumor hypoxia blocks Wnt processing and secretion through the induction of endoplasmic reticulum stress. Mol Cell Biol 28:7212-24 (2008).
WB, IP;
Mouse.
PubMed: 18824543
- Masud A et al. Endoplasmic reticulum stress-induced death of mouse embryonic fibroblasts requires the intrinsic pathway of apoptosis. J Biol Chem 282:14132-9 (2007).
PubMed: 17371867
- Yang LP et al. Activation of endoplasmic reticulum stress in degenerating photoreceptors of the rd1 mouse. Invest Ophthalmol Vis Sci 48:5191-8 (2007).
PubMed: 17962473
Publishing research using ab21685? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GRP78 BiP antibody (ab21685)
All lanes : Anti-GRP78 BiP antibody (ab21685) at 1 µg/ml
Lane 1 : CHO-K1 whole cell lysate at 20 µg
Lane 2 : Liver (Mouse) Tissue Lysate at 20 µg
Lane 3 : Rat liver whole cell lysate at 20 µg
Lane 4 : HeLa whole cell lysate at 20 µg
Lane 5 : CHO-K1 whole cell lysate at 20 µg/ml with GRP78 BiP peptide (ab22410) at 1 µg
Lane 6 : Liver (Mouse) Tissue Lysate at 20 µg with GRP78 BiP peptide (ab22410) at 1 µg/ml
Lane 7 : Rat liver whole cell lysate at 20 µg with GRP78 BiP peptide (ab22410) at 1 µg/ml
Lane 8 : HeLa whole cell lysate at 20 µg with GRP78 BiP peptide (ab22410) at 1 µg/ml
Secondary
Goat anti Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 78 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
ab21685 recognises a band of ~ 75 kDa in CHO, mouse liver, rat liver and HeLa whole cell lysates, corresponding to GRP78 BiP. This band is quenched by the addition of the immunizing peptide, ab22410.
ab21685 also detects a 100 kDa band in Western Blot. We are unsure of the identity of this protein.
Immunocytochemistry/ Immunofluorescence - GRP78 BiP antibody (ab21685)
ICC/IF image of ab21685 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21685, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunocytochemistry/ Immunofluorescence - GRP78 BiP antibody (ab21685)
ICC/IF image of ab21685 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21685, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP78 BiP antibody (ab21685)
IHC image of GRP78 BiP staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21685, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - GRP78 BiP antibody (ab21685)
All lanes : Anti-GRP78 BiP antibody (ab21685) at 1 µg/ml
Lane 1 : CHO-K1 cell lysate Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate at 10 µg
Lane 3 : Liver (Rat) Tissue Lysate at 10 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 78 kDa
Observed band size : 78 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - GRP78 BiP antibody (ab21685)
ab21685 at a 1/500 dilution staining GRP78 BiP in mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C. PFA fixed. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at a 1/500 dilution polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488 (green). Nuclei were counterstained with DAPI (blue).
This image was kindly supplied by Dr Vladimir Milenkovic by Abreview
Immunocytochemistry/ Immunofluorescence - GRP78 BiP antibody (ab21685)
ab21685 at a 1/500 dilution staining GRP78 BiP in Dog MDCK II cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4ºC. PFA fixed. Blocked with 5% serum for 20 minutes at 25ºC. Secondary used at a 1/500 dilution polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488 (green). Nuclei were counterstained with DAPI (blue).
This image was kindly supplied by Dr Vladimir Milenkovic by Abreview
Immunocytochemistry/ Immunofluorescence - GRP78 BiP antibody (ab21685)
ICC/IF image of ab21685 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21685, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot
All lanes : Anti-GRP78 BiP antibody (ab21685) at 1 µg/ml
Lane 1 : GRP78 BiP protein (Active) (ab78432) at 0.1 µg
Lane 2 : GRP78 BiP protein (Active) (ab78432) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
GRP78 BiP antibody for WB in Human (21685)
GRP78 BiP antibody for Western blot in Mouse (21685)
GRP78 BiP antibody for Western blot in Mouse (21685)
GRP78 BiP antibody for Western blot in Human (21685)
Anti-GRP78 BiP antibody for Western blot in Human (21685)
GRP78 BiP antibody for ICC/IF in Mouse (21685)
GRP78 BiP antibody for ICC/IF in Human (21685)
GRP78 BiP antibody for ICC/IF in Dog (21685)
GRP78 BiP antibody for ICC/IF in Rat (21685)
GRP78 BiP antibody for Immunoprecipitation in Human (21685)
GRP78 BiP antibody for ICC/IF in Mouse (21685)
GRP78 BiP antibody for ICC/IF in Human (21685)
Anti-GRP78 BiP antibody for Immunocytochemistry/ Immunofluorescence in Human (21685)
Anti-GRP78 BiP antibody for Immunoprecipitation in Human (21685)
Anti-GRP78 BiP antibody for Western blot in Human (21685)
GRP78 BiP antibody for Western blot in Mouse (21685)
GRP78 BiP antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (21685)
GRP78 BiP antibody for Immunocytochemistry/ Immunofluorescence in Mouse (21685)
GRP78 BiP antibody for ICC/IF in Pig (21685)
GRP78 BiP antibody for WB in Human (21685)
GRP78 BiP antibody for WB in Human (21685)
GRP78 BiP antibody for WB in Rat (21685)
Anti-GRP78 BiP antibody for Western blot in Mouse (21685)
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