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Microtomy (Formalin-fixed paraffin-embedded sections) - GRP78 BiP antibody (ab32618)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GRP78 BiP antibody(ab32618)
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Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab32618)
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Product Name
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GRP78 BiP antibody
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See all GRP78 BiP - ER marker antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GRP78 BiP
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Immunogen
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Synthetic peptide: KEDVGTV VGIDLGTTYS CVG, corresponding to amino acids 24-43 of Human GRP78 BiP
BLAST 'KEDVGTV VGIDLGTTYS CVG' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Hm, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells, breast carcinoma.
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Cellular localization
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Endoplasmic reticulum lumen.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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Relevance
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The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GRP78 BiP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GRP78 BiP antibody images, references, products related to ab32618 and other tools.
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GRP78 BiP antibody images:
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 Microtomy (Formalin-fixed paraffin-embedded sections) - GRP78 BiP antibody (ab32618)
This image shows human breast carcinoma stained with ab32618 diluted 1/100.
This image shows human breast carcinoma stained with ab32618 diluted 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GRP78 BiP antibody(ab32618)
Ab32618 staining Human normal liver parenchyma. Staining is localised to endoplasmic reticulum compartment.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab32618)
ICC/IF image of ab32618 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32618, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for GRP78 BiP antibody (ab32618)
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This product has been used in: (two most recent references)
Hempel F et al. ERAD-derived preprotein transport across the second outermost plastid membrane of diatoms. Mol Biol Evol 26:1781-90 (2009). WB. PubMed: 19377060
Macedo B et al. Anti-apoptotic treatment reduces transthyretin deposition in a transgenic mouse model of Familial Amyloidotic Polyneuropathy. Biochim Biophys Acta 1782:517-22 (2008). WB; Mouse. PubMed: 18572024
If you publish research using ab32618 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GRP78 BiP
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GRP78 BiP antibody - more information
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GRP78 BiP antibody - other tools:
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