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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - GRP78 BiP antibody (ab53068)
All lanes : Anti-GRP78 BiP antibody (ab53068) at 1/300 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with immunising peptide
Predicted band size : 72 kDa
Observed band size : 72 kDa
Immunohistochemistry (Paraffin-embedded sections) - GRP78 BiP antibody (ab53068)
ab53068 at 1/50 dilution staining GRP78 BiP in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab53068)
ICC/IF image of ab53068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53068, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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