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Western blot - GRP78 BiP antibody (ab53068)
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Immunohistochemistry (Paraffin-embedded sections) - GRP78 BiP antibody (ab53068)
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Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab53068)
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Product Name
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GRP78 BiP antibody
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See all GRP78 BiP - ER marker antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GRP78 BiP
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Immunogen
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A synthetic peptide derived from human GRP78 BiP.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab53068 detects endogenous levels of total GRP78 BiP protein.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/300 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue and COS7 cell extracts.
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Cellular localization
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Endoplasmic reticulum lumen.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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Relevance
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The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab53068 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GRP78 BiP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GRP78 BiP antibody images, references, products related to ab53068 and other tools.
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GRP78 BiP antibody images:
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 Western blot - GRP78 BiP antibody (ab53068)
All lanes : GRP78 BiP antibody (ab53068) at 1/300 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with immunising peptide
Predicted band size : 72 kDa
Observed band size : 72 kDa
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Immunohistochemistry (Paraffin-embedded sections) - GRP78 BiP antibody (ab53068)
ab53068 at 1/50 dilution staining GRP78 BiP in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab53068)
ICC/IF image of ab53068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to GRP78 BiP
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GRP78 BiP antibody - more information
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Customer reviews (feedback) regarding GRP78 BiP antibody |
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