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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Overview
Product name
Anti-GRP78 BiP antibody
See all GRP78 BiP products (13) ...
Description
Rabbit polyclonal to GRP78 BiP
Specificity
ab53068 detects endogenous levels of total GRP78 BiP protein.
Tested applications
IHC-P, ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human)(Peptide available as ab106845.)
Positive control
Human breast carcinoma tissue and COS7 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53068 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/300 - 1/1000.Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Target
Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Involvement in disease
Note=Autoantigen in rheumatoid arthritis.
Sequence similarities
Belongs to the heat shock protein 70 family.
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP11021
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- BIP antibody
see all
Database links
- Entrez Gene: 3309 Human
- Entrez Gene: 14828 Mouse
- Entrez Gene: 25617 Rat
- Omim: 138120 Human
- SwissProt: P11021 Human
- SwissProt: P20029 Mouse
- SwissProt: P06761 Rat
- Unigene: 605502 Human
see all
Anti-GRP78 BiP antibody images:
Western blot - GRP78 BiP antibody (ab53068)
All lanes : Anti-GRP78 BiP antibody (ab53068) at 1/300 dilution
Lane 1 : COS7 cell extract
Lane 2 : COS7 cell extract with immunising peptide
Predicted band size : 72 kDa
Observed band size : 72 kDa
Immunohistochemistry (Paraffin-embedded sections) - GRP78 BiP antibody (ab53068)
ab53068 at 1/50 dilution staining GRP78 BiP in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence-GRP78 BiP antibody(ab53068)
ICC/IF image of ab53068 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GRP78 BiP antibody (ab53068)
This product has been referenced in:
- Spencer NYet al. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 286:8977-87 (2011). WB; Mouse.Read more (PubMed: 21212270) »
- Vanderperre Bet al. An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB J 25:2373-86 (2011).Read more (PubMed: 21478263) »
See all 3 publications for this product
Publishing research using ab53068? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"