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- Secondary antibodies
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Product name
Anti-GRP94 antibody [9G10] - ER Marker
See all GRP94 products (17) ...
Description
Rat monoclonal [9G10] to GRP94 - ER Marker
Tested applications
WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Hamster, Cow
Immunogen
Chick oviduct GRP 94
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
9G10
Isotype
IgG2a
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Protein Trafficking >> ER Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC/IF
IP
Application notes
Immunocytochemistry/Immunofluorescence: 1/250
Western Blot: 1/5000
Immunoprecipitation: Assay dependent.
Not tested in other applications
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects an ~108 kDa protein representing GRP94 in chick oviduct cytosol.
Target
Function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence similarities
Belongs to the heat shock protein 90 family.
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP14625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Database links
- Entrez Gene: 374163 Chicken
- Entrez Gene: 282646 Cow
- Entrez Gene: 22027 Mouse
- Entrez Gene: 362862 Rat
- SwissProt: P08110 Chicken
- SwissProt: Q95M18 Cow
- SwissProt: P08712 Hamster
- SwissProt: P08113 Mouse
see all
References for Anti-GRP94 antibody [9G10] - ER Marker (ab2791)
This product has been referenced in:
- Gething MJ & Sambrook J Protein folding in the cell. Nature 355:33-45 (1992).Read more (PubMed: 1731198) »
- Kulomaa MS et al. Amino acid sequence of a chicken heat shock protein derived from the complementary DNA nucleotide sequence. Biochemistry 25:6244-51 (1986).Read more (PubMed: 3024703) »
See all 4 publications for this product
Publishing research using ab2791? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"