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Product name
Anti-GRP94 antibody - ER Marker
See all GRP94 products (17) ...
Description
Rabbit polyclonal to GRP94 - ER Marker
Tested applications
ICC, IF, WBmore details
Cross reactivity
Reacts with
Hamster, Human, Monkey
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide (Human) (Internal sequence).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Protein Trafficking >> ER Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
IF
Application notes
ICC: 1/100 - 1/400.
IF: 1/100 - 1/400.
WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Works in ICC/IF with methanol-fixed and 4% paraformaldehyde fixed cells.
Target
Function
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence similarities
Belongs to the heat shock protein 90 family.
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP14625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 94 kDa glucose regulated protein antibody
- 94 kDa glucose-regulated protein antibody
- ECGP antibody
see all
Database links
- Entrez Gene: 7184 Human
- Omim: 191175 Human
- SwissProt: P08712 Hamster
- SwissProt: P14625 Human
- Unigene: 192374 Human
Anti-GRP94 antibody - ER Marker images:
Immunocytochemistry/ Immunofluorescence - GRP94 antibody - ER Marker (ab18055)
Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.
Western blot - GRP94 antibody - ER Marker (ab18055)
Predicted band size : 94 kDa
Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.
References for Anti-GRP94 antibody - ER Marker (ab18055)
This product has been referenced in:
- Mulya A et al. Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1. J Lipid Res 51:514-24 (2010). ICC/IF; Human.Read more (PubMed: 19767535) »
- Kang BY et al. Differential gene expression profiling in HELLP syndrome placentas. Reprod Sci 15:285-94 (2008).Read more (PubMed: 18421023) »
See all 2 publications for this product
Publishing research using ab18055? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"