Anti-GRP94 antibody [SPM249], prediluted (ab54244)
Overview
- Product nameAnti-GRP94 antibody [SPM249], predilutedSee all GRP94 primary antibodies ...
- DescriptionRat monoclonal [SPM249] to GRP94, prediluted
- Tested applicationsIHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human, Monkey
Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis
- Immunogen
Purified glucose regulated protein 94 (GRP94) from chicken oviducts.
- Positive controlSKBR-3 cells and tonsil tissue.
Properties
- FormPrediluted
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityProtein G purified
- Clonality Monoclonal
- Clone numberSPM249
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab54244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P |
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
- Sequence similaritiesBelongs to the heat shock protein 90 family.
- Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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Database links
- Entrez Gene: 374163 Chicken
- Entrez Gene: 282646 Cow
- Entrez Gene: 404019 Dog
- Entrez Gene: 7184 Human
- Entrez Gene: 22027 Mouse
- Entrez Gene: 397191 Pig
- Entrez Gene: 100009456 Rabbit
- Entrez Gene: 362862 Rat
- Omim: 191175 Human
- SwissProt: P08110 Chicken
- SwissProt: Q95M18 Cow
- SwissProt: P41148 Dog
- SwissProt: P14625 Human
- SwissProt: P08113 Mouse
- SwissProt: Q29092 Pig
- SwissProt: O18750 Rabbit
- SwissProt: Q66HD0 Rat
- Unigene: 192374 Human
- Unigene: 87773 Mouse
- Unigene: 101146 Rat
see all
Target information above from: UniProt accession
P14625
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 94 kDa glucose regulated protein antibody94 kDa glucose-regulated protein antibodyECGP antibody
- Endoplasmin antibodyEndothelial cell (HBMEC) glycoprotein antibodyENPL_HUMAN antibodyGlucose regulated protein 94kDa antibodygp96 antibodygp96 homolog antibodyGRP 94 antibodyGRP-94 antibodyHeat shock protein 90 kDa beta member 1 antibodyheat shock protein 90kDa beta (Grp94), member 1 antibodyHeat shock protein, 90 kDa, beta, 1 antibodyHSP90B1 antibodyStress inducible tumor rejection antigen GP96 antibodyTRA1 antibodytumor rejection antigen (gp96) 1 antibodyTumor rejection antigen 1 antibodyTumor rejection antigen gp96 antibodyTumor rejection antigen-1 (gp96) antibody
see all
Anti-GRP94 antibody [SPM249], prediluted images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP94 antibody [SPM249], prediluted (ab54244)](http://a.abcam.com/ps/datasheet/Images/54/ab54244/ab54244_1.bmp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GRP94 antibody [SPM249], prediluted (ab54244)ab54244 staining human GRP94 in human tonsil tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
References for Anti-GRP94 antibody [SPM249], prediluted (ab54244)
This product has been referenced in:
- Leemans JC et al. The role of Toll-like receptor 2 in inflammation and fibrosis during progressive renal injury. PLoS One 4:e5704 (2009). IHC-P ; Mouse . Read more (PubMed: 19479087) »
