Overview
- Product nameAnti-GSK3 alpha antibody [6G2F1,6G2F2]See all GSK3 alpha primary antibodies ...
- DescriptionMouse monoclonal [6G2F1,6G2F2] to GSK3 alpha
- Tested applicationsWB, ELISA, IHC-Fr, IHC-P more details
- Species reactivityReacts with: Recombinant Fragment
Predicted to work with: Human
- Immunogen
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E.coli strain BL21 (DE3)
- Positive controlA459 cell lysate (WB). Human skin and lung carcinomas (IHC-P).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone number6G2F1,6G2F2
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab59955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
| ELISA | ELISA: 1/10000. |
| IHC-Fr | IHC-Fr: 1/500 - 1/2000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Database links
- Entrez Gene: 2931 Human
- Entrez Gene: 2931 Human
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: P49840 Human
- Unigene: 466828 Human
Target information above from: UniProt accession
P49840
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- DKFZp686D0638 antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase-3 alpha antibody
- GSK 3 alpha antibodyGSK 3A antibodyGSK-3 alpha antibodyGSK3A antibodyGSK3A_HUMAN antibodyGSK3alpha antibodySerine/threonine protein kinase GSK3A antibody
see all
Anti-GSK3 alpha antibody [6G2F1,6G2F2] images
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![Western blot - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)](http://a.abcam.com/ps/datasheet/Images/59/ab59955/ab59955wb.jpg)
Western blot - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)All lanes : Anti-GSK3 alpha antibody [6G2F1,6G2F2] (ab59955) at 1/500 dilution
Lane 1 : Molecular weight markers
Lane 2 : Truncated GSK3 alpha recombinant protein
Lane 3 : A549 cell lysate
Predicted band size : 51 kDa
Observed band size : 51 kDa -
![Immunohistochemistry (Paraffin-embedded sections) - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)](http://a.abcam.com/ps/datasheet/Images/59/ab59955/ab59955ihc1.jpg)
Immunohistochemistry (Paraffin-embedded sections) - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)ab59955, at 1/500 dilution, staining human GSK3 alpha in lung carcinoma with DAB staining. Paraffin embedded tissue. -
![Immunohistochemistry (Paraffin-embedded sections) - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)](http://a.abcam.com/ps/datasheet/Images/59/ab59955/ab59955ihc2.jpg)
Immunohistochemistry (Paraffin-embedded sections) - GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)ab59955, at 1/500 dilution, staining human GSK3 alpha in skin carcinoma with DAB staining; Paraffin embedded tissue.
References for Anti-GSK3 alpha antibody [6G2F1,6G2F2] (ab59955)
ab59955 has not yet been referenced specifically in any publications.
