Anti-GSK3 alpha antibody [9D5G1] (ab54221)

Overview

• Product nameAnti-GSK3 alpha antibody [9D5G1]See all GSK3 alpha primary antibodies ...
• Description
  Mouse monoclonal [9D5G1] to GSK3 alpha
• Tested applicationsWB, ELISA, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Ni-NTA purified truncated recombinant fragment of GSK3 alpha (Human) expressed in E.coli strain BL21 (DE3)

• Positive controlHela cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number9D5G1
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Cardiovascular
  • Heart
  • Apoptosis

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
  Contains 1 protein kinase domain.

Target information above from: UniProt accession P49840 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2931 Human
  • Entrez Gene: 2931 Human
  • Omim: 606784 Human
  • SwissProt: P49840 Human
  • SwissProt: P49840 Human
  • Unigene: 466828 Human

• Alternative names
  DKFZp686D0638 antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase-3 alpha antibody

  GSK 3 alpha antibodyGSK 3A antibodyGSK-3 alpha antibodyGSK3A antibodyGSK3A_HUMAN antibodyGSK3alpha antibodySerine/threonine protein kinase GSK3A antibody

  see all

Anti-GSK3 alpha antibody [9D5G1] images

• 

  Western blot - GSK3 alpha antibody [9D5G1] (ab54221)
  Lane 1 : Anti-GSK3 alpha antibody [9D5G1] (ab54221)
  Lane 2 : Anti-GSK3 alpha antibody [9D5G1] (ab54221) at 1/500 dilution
  
  Lane 1 : Molecular weight marker
  Lane 2 : Truncated GSK3 alpha recombinant protein
  
  
  Predicted band size : 51 kDa
  Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - GSK3 alpha antibody [9D5G1] (ab54221)
  Immunocytochemistry / Immunofluorescence analysis of Hela cells using ab54221 (1/200 dilution) showing cytoplasmic localization.