Overview

  • Product nameAnti-GSK3 alpha antibody [9D5G1]See all GSK3 alpha primary antibodies ...
  • Description
    Mouse monoclonal [9D5G1] to GSK3 alpha
  • Tested applicationsWB, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ni-NTA purified truncated recombinant fragment of GSK3 alpha (Human) expressed in E.coli strain BL21 (DE3)

  • Positive control
    • Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab54221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/2000. Predicted molecular weight: 51 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P49840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686D0638 antibody
    • Glycogen synthase kinase 3 alpha antibody
    • Glycogen synthase kinase-3 alpha antibody
    • GSK 3 alpha antibody
    • GSK 3A antibody
    • GSK-3 alpha antibody
    • GSK3A antibody
    • GSK3A_HUMAN antibody
    • GSK3alpha antibody
    • Serine/threonine protein kinase GSK3A antibody
    see all

Anti-GSK3 alpha antibody [9D5G1] images

  • Lane 1 : Anti-GSK3 alpha antibody [9D5G1] (ab54221)
    Lane 2 : Anti-GSK3 alpha antibody [9D5G1] (ab54221) at 1/500 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Truncated GSK3 alpha recombinant protein


    Predicted band size : 51 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry / Immunofluorescence analysis of Hela cells using ab54221 (1/200 dilution) showing cytoplasmic localization.

References for Anti-GSK3 alpha antibody [9D5G1] (ab54221)

ab54221 has not yet been referenced specifically in any publications.

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