Overview

  • Product nameAnti-GSK3 alpha antibodySee all GSK3 alpha primary antibodies ...
  • Description
    Rabbit polyclonal to GSK3 alpha
  • Tested applicationsWB, ELISA, IHC-P, IP, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human GSK3 around the phosphorylation site of Serine 21.

  • Positive control
    • WB: extracts from ovary cancer cells or K562/HepG2/RAW264.7 cells IHC-P: breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab28833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
IHC-P
IP
ICC/IF
  • Application notesELISA: 1/20000.
    ICC/IF: 1/500 (PMID 19858210).
    IHC-P: 1/50 - 1/100.
    IP: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 51 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
      Contains 1 protein kinase domain.
    • Target information above from: UniProt accession P49840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686D0638 antibody
      • Glycogen synthase kinase 3 alpha antibody
      • Glycogen synthase kinase-3 alpha antibody
      • GSK 3 alpha antibody
      • GSK 3 alpha antibody
      • GSK 3A antibody
      • GSK-3 alpha antibody
      • GSK3A antibody
      • GSK3A_HUMAN antibody
      • GSK3alpha antibody
      • Serine/threonine protein kinase GSK3A antibody
      see all

    Anti-GSK3 alpha antibody images

    • Ab28833, at a dilution of 1/50, staining GSK3 alpha in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
      Left image: untreated.
      Right image: preincubated with synthesized peptide.
    • All lanes : Anti-GSK3 alpha antibody (ab28833) at 1/500 dilution

      Lane 1 : Line 1: analysis of extracts from ovary cancer cells (5-30ug) using ab28833
      Lane 2 : Line 2: analysis of extracts from ovary cancer cells (5-30ug) using ab28833 preincubated with synthesized peptide


      Predicted band size : 51 kDa
      Observed band size : 51 kDa
    • Anti-GSK3 alpha antibody (ab28833) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells

      Secondary
      HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 51 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • ab28833 staining GSK3 alpha in HeLa cells by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 0.5% Triton-X prior to blocking in 3% serum for 1 hour at 23°C. The primary antibody was diluted 1/100 in PBS-BSA (3%) and incubated with the sample for 1 hour at 23°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.

      See Abreview

    References for Anti-GSK3 alpha antibody (ab28833)

    This product has been referenced in:
    • Kapfhamer D  et al. Protein Phosphatase 2a and glycogen synthase kinase 3 signaling modulate prepulse inhibition of the acoustic startle response by altering cortical M-Type potassium channel activity. J Neurosci 30:8830-40 (2010). WB ; Mouse . Read more (PubMed: 20592205) »
    • Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB ; Human . Read more (PubMed: 20932480) »

    See all 6 Publications for this product

    Product Wall

    Thank you for contacting us.

    I can confirm, that the Anti-GSK3 alpha antibody (ab28833) will detect endogenous levels of total GSK3 alpha protein. It should for this reason detect the non-phosphorylated as well as the phosphorylated forms of G...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (MEF)
    Specification MEF
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X
    Username

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    Verified customer

    Submitted Mar 07 2011

    Application Western blot
    Sample Human Cell lysate - other (HELA)
    Loading amount 50 µg
    Specification HELA
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

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    Verified customer

    Submitted Feb 22 2011

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (HELA)
    Specification HELA
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 15 2011

    Application Western blot
    Sample Human Cell lysate - whole cell (jurkat cells)
    Loading amount 200000 cells
    Specification jurkat cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 24 2009

    Thank you for your enquiry. To my knowledge, there is no experimental data to indicate if this antibody (ab28833) will detect GSK3 beta. However, I have done a BLAST sequence alignment search and there is 15% sequence homology between the immunogen ...

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