Overview

  • Product name
  • Description
    Rabbit polyclonal to GSK3 alpha
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GSK3 alpha.

    (Peptide available as ab93065.)

  • Positive control
    • Ab78664 gave a positive signal in the following whole cell lysates: HeLa; A549; HEK293. This antibody also gave a positive signal in Mouse and Rat Brain Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab78664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).

Target

Images



  • Predicted band size : 50 kDa

    Lane 1: GSK3A knockout HAP1 whole cell lysate (20 µg)
    Lane 2: Wild type HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab78664 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab78664 was shown to recognize GSK3A when GSK3A knockout samples were used, along with additional cross-reactive bands. Wild-type and GSK3A knockout samples were subjected to SDS-PAGE.  Followed by incubation with ab78664 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a 1/250 and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

References

ab78664 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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