Overview

  • Product nameAnti-GSK3 alpha antibody
    See all GSK3 alpha primary antibodies
  • Description
    Rabbit polyclonal to GSK3 alpha
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GSK3 alpha.

    (Peptide available as ab93065.)

  • Positive control
    • Ab78664 gave a positive signal in the following whole cell lysates: HeLa; A549; HEK293. This antibody also gave a positive signal in Mouse and Rat Brain Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab78664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-GSK3 alpha antibody images

    • All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 50 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 minutes
    • All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 50 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    References for Anti-GSK3 alpha antibody (ab78664)

    ab78664 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab78664.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"