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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes WB: 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Sequence similarities Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.
Information by UniProt
Glycogen synthase kinase 3 alpha antibody
Glycogen synthase kinase-3 alpha antibody
Anti-GSK3 alpha antibody images
Western blot - GSK3 alpha antibody (ab78664)
All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa ( why is the actual band size different from the predicted?) Exposure time : 20 minutes
Western blot - Anti-GSK3 alpha antibody (ab78664)
All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 55 kDa ( why is the actual band size different from the predicted?) Exposure time : 3 minutes
References for Anti-GSK3 alpha antibody (ab78664)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"