Anti-GSK3 alpha antibody (ab78664)

Overview

• Product nameAnti-GSK3 alpha antibodySee all GSK3 alpha primary antibodies ...
• Description
  Rabbit polyclonal to GSK3 alpha
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human GSK3 alpha.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab93065.)

• Positive controlAb78664 gave a positive signal in the following whole cell lysates: HeLa; A549; HEK293. This antibody also gave a positive signal in Mouse and Rat Brain Tissue Lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Cardiovascular
  • Heart
  • Apoptosis

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
  Contains 1 protein kinase domain.

Target information above from: UniProt accession P49840 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2931 Human
  • Entrez Gene: 2931 Human
  • Entrez Gene: 606496 Mouse
  • Entrez Gene: 50686 Rat
  • Omim: 606784 Human
  • SwissProt: P49840 Human
  • SwissProt: P49840 Human
  • SwissProt: Q2NL51 Mouse

  • SwissProt: P18265 Rat
  • Unigene: 466828 Human
  • Unigene: 294664 Mouse
  • Unigene: 36807 Rat

  see all

• Alternative names
  DKFZp686D0638 antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase-3 alpha antibody

  GSK 3 alpha antibodyGSK 3A antibodyGSK-3 alpha antibodyGSK3A antibodyGSK3A_HUMAN antibodyGSK3alpha antibodySerine/threonine protein kinase GSK3A antibody

  see all

Anti-GSK3 alpha antibody images

• 

  Western blot - GSK3 alpha antibody (ab78664)
  All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
  Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 50 kDa
  Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 20 minutes
• 

  Western blot - Anti-GSK3 alpha antibody (ab78664)
  All lanes : Anti-GSK3 alpha antibody (ab78664) at 1/250 dilution
  
  Lane 1 : Brain (Mouse) Tissue Lysate
  Lane 2 : Brain (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 50 kDa
  Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 3 minutes