Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)
- Product nameAnti-GSK3 (alpha + beta) antibody [15.4]See all GSK3 (alpha + beta) primary antibodies ...
- DescriptionRabbit monoclonal [15.4] to GSK3 (alpha + beta)
- Specificityab78277 recognizes both GSK3 alpha and beta.
- Tested applicationsWB, IP more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length human GSK3 beta with N-terminal 6 His-tag, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.
- Positive control
- A431, HEK293, Jurkat, and 3T3/A31 cell lysates. HeLa Nuclear lysate.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 70% Storage buffer (0.15M Sodium chloride, 0.1M Tris glycine, pH 7.4)
- Concentration information loading...
- PurityProtein A purified
- Clonality Monoclonal
- Clone number15.4
- Research Areas
Our Abpromise guarantee covers the use of ab78277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/20000. Detects bands of approximately 47 and 51 kDa. Predicted molecular weights 47 kDa (beta) and 51 kDa (alpha).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Cellular localizationCytoplasmic and Nuclear
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: P49841 Human
- SwissProt: Q2NL51 Mouse
- SwissProt: Q9WV60 Mouse
- SwissProt: P18265 Rat
- SwissProt: P18266 Rat
- Unigene: 445733 Human
- Unigene: 466828 Human
- Unigene: 10426 Rat
- Unigene: 36807 Rat
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Anti-GSK3 (alpha + beta) antibody [15.4] images
Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277) at 1/5000 dilution + HEK293 cell lysate
Goat anti-rabbit HRP conjugate
Predicted band size : 47 , 51 kDa
Observed band size : 47,51 kDa
References for Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)
ab78277 has not yet been referenced specifically in any publications.