You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.
RabMAbs

Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)

Submit an Abreview Submit a Question

Overview

  • Product nameAnti-GSK3 (alpha + beta) antibody [15.4]See all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Rabbit monoclonal [15.4] to GSK3 (alpha + beta)
  • Specificityab78277 recognizes both GSK3 alpha and beta.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length human GSK3 beta with N-terminal 6 His-tag, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

  • Positive controlA431, HEK293, Jurkat, and 3T3/A31 cell lysates. HeLa Nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab78277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: 2 - 8µl/100µg of lysate.
    WB: 1/1000 - 1/20000. Detects bands of approximately 47 and 51 kDa. Predicted molecular weights 47 kDa (beta) and 51 kDa (alpha).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
      see all
    • Alternative names
        C86142 antibodyDKFZp686D0638 antibodyFA antibody
        Factor A antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibodyGSK 3 alpha antibodyGSK 3 antibodyGSK 3 beta antibodyGSK 3A antibodyGSK 3B antibodyGSK3 alpha antibodyGSK3 beta antibodyGsk3a antibodyGSK3B antibodyKG3A antibodyKG3B antibody
      see all

    Anti-GSK3 (alpha + beta) antibody [15.4] images

    • Western blot - GSK3 (alpha + beta) antibody (ab78277)
      Western blot - GSK3 (alpha + beta) antibody (ab78277)
      Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277) at 1/5000 dilution + HEK293 cell lysate

      Secondary
      Goat anti-rabbit HRP conjugate

      Predicted band size : 47 , 51 kDa
      Observed band size : 47,51 kDa

    References for Anti-GSK3 (alpha + beta) antibody [15.4] (ab78277)

    ab78277 has not yet been referenced specifically in any publications.

    Publishing research using ab78277 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab78277 Submit a Question for ab78277

    There are currently no Abreviews or Questions for ab78277.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"