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Overview

  • Product nameAnti-GSK3 (alpha + beta) antibody [M132]See all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279)
  • SpecificityThis antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3ß and GSK-3a
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A phospho-GSK-3ß (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3ß.

  • Positive controlPervanadate treated rabbit fibroblasts and treated human SKN-SH and A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab45383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000. Predicted molecular weight: 52 kDa.
ELISA ELISA: 1/2000.

Target

  • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
    see all
  • Alternative names
      C86142 antibodyDKFZp686D0638 antibodyFA antibody
      Factor A antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibodyGSK 3 alpha antibodyGSK 3 antibodyGSK 3 beta antibodyGSK 3A antibodyGSK 3B antibodyGSK3 alpha antibodyGSK3 beta antibodyGsk3a antibodyGSK3B antibodyKG3A antibodyKG3B antibody
    see all

Anti-GSK3 (alpha + beta) antibody [M132] images

  • Western blot - GSK3 (alpha + beta) antibody [M132] (ab45383)
    Western blot - GSK3 (alpha + beta) antibody [M132] (ab45383)
    Lanes 1 - 2 : anti-GSK-3ß
    Lanes 3 - 4 : Anti-GSK3 (alpha + beta) antibody [M132] (ab45383) at 1/1000 dilution

    Lane 1 : rabbit spleen fibroblasts serum starved for 2 hrs
    Lane 2 : rabbit spleen fibroblasts treated with pervanadate (1 mM) for 30 minutes
    Lane 3 : rabbit spleen fibroblasts serum starved for 2 hrs
    Lane 4 : rabbit spleen fibroblasts treated with pervanadate (1 mM) for 30 minutes


    Predicted band size : 52 kDa
    Observed band size : 46,50 kDa (why is the actual band size different from the predicted?)

References for Anti-GSK3 (alpha + beta) antibody [M132] (ab45383)

This product has been referenced in:
  • Puri P  et al. Proteomic Analysis of Bovine Sperm YWHA Binding Partners Identify Proteins Involved in Signaling and Metabolism. Biol Reprod : (2008). WB ; Cow . Read more (PubMed: 18753613) »

See 1 Publication for this product

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