Anti-GSK3 (alpha + beta) antibody (ab15314)

Overview

• Product nameAnti-GSK3 (alpha + beta) antibodySee all GSK3 (alpha + beta) primary antibodies ...
• Description
  Rabbit polyclonal to GSK3 (alpha + beta)
• Tested applicationsICC, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Monkey
• Immunogen

  Synthetic peptide (Human) (C terminal).

• Positive controlJurkat cells and brain
• General notes

  
  

  GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3ß. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Primary antibody notes GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3ß. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 2932 Human
  • Entrez Gene: 2931 Human
  • Entrez Gene: 56637 Mouse
  • Entrez Gene: 606496 Mouse
  • Entrez Gene: 84027 Rat
  • Entrez Gene: 50686 Rat
  • Omim: 605004 Human
  • Omim: 606784 Human

  • SwissProt: P49840 Human
  • SwissProt: P49841 Human
  • SwissProt: Q9WV60 Mouse
  • SwissProt: Q2NL51 Mouse
  • SwissProt: P18265 Rat
  • SwissProt: P18266 Rat
  • Unigene: 466828 Human
  • Unigene: 445733 Human
  • Unigene: 36807 Rat
  • Unigene: 10426 Rat

  see all

• Alternative names
  C86142 antibodyDKFZp686D0638 antibodyFA antibody

  Factor A antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibodyGlycogen synthase kinase 3 beta antibodyGSK 3 alpha antibodyGSK 3 antibodyGSK 3 beta antibodyGSK 3 beta antibodyGSK 3A antibodyGSK 3B antibodyGSK3 alpha antibodyGSK3 beta antibodyGsk3a antibodyGSK3B antibodyKG3A antibodyKG3B antibody

  see all

Anti-GSK3 (alpha + beta) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 (alpha + beta) antibody (ab15314)
  ab15314 (1/100) staining GSK3 in formalin fixed, paraffin-embbeded human brain tissue sections. Antigen retrieval was performed by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
• 

  Immunocytochemistry - Anti-GSK3 (alpha + beta) antibody (ab15314)Image from Wozniak MA et al., PLoS One. 2011;6(10):e25152. Epub 2011 Oct 7. Fig 10.; doi:10.1371/journal.pone.0025152; October 7, 2011, PLoS ONE 6(10): e25152.
  ab15314 staining GSK3 (alpha + beta) in Vero cells infected with HSV1 by Immunocytochemistry.
  
  Cells were infected with HSV1 SC16 at an MOI of 1 for 16 hours, fixed with formaldehyde and blocked by TBS containing 0.025% Triton X-100. Samples were incubated with primary antibody (1/100 in diluent) for 10 minutes.