Anti-GSK3 (alpha + beta) antibody (ab52179)
- Product nameAnti-GSK3 (alpha + beta) antibodySee all GSK3 (alpha + beta) primary antibodies ...
- DescriptionRabbit polyclonal to GSK3 (alpha + beta)
- Specificityab52179 detects endogenous levels of total GSK3a/ß protein.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human GSK3a/ß around the phosphorylation site of tyrosine 279/216 (V-S-Y-I-C).
- Positive control
- Extracts from 293 cells, treated with TNF-a (20ng/ml, 30min).
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab52179 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab52179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
- RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Cellular localizationCytoplasmic and Nuclear
- Entrez Gene: 2931 Human
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 50686 Rat
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: P49841 Human
- SwissProt: Q2NL51 Mouse
- SwissProt: Q9WV60 Mouse
- SwissProt: P18265 Rat
- SwissProt: P18266 Rat
- Unigene: 445733 Human
- Unigene: 466828 Human
- Unigene: 10426 Rat
- Unigene: 36807 Rat
- Factor A antibody
- Glycogen synthase kinase 3 alpha antibody
- Glycogen synthase kinase 3 beta antibody
- GSK3 alpha antibody
- GSK3 beta antibody
- GSK3B antibody
Anti-GSK3 (alpha + beta) antibody images
All lanes : Anti-GSK3 (alpha + beta) antibody (ab52179) at 1/500 dilution
Lane 1 : 293 cell extract, treated with TNF-a (20ng/ml, 30min)
Lane 2 : 293 cell extract, treated with TNF-a (20ng/ml, 30min) with immunising peptide
Predicted band size : 52 kDa
Observed band size : 49,52 kDa (why is the actual band size different from the predicted?)