Anti-GSK3 (alpha + beta) antibody (ab82542)

Overview

• Product nameAnti-GSK3 (alpha + beta) antibodySee all GSK3 (alpha + beta) primary antibodies ...
• Description
  Rabbit polyclonal to GSK3 (alpha + beta)
• SpecificityRecognises both GSK3 alpha and GSK3 beta.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide, corresponding to amino acids 214-218 of Human GSK3 beta

• Positive controlJurkat cell lysate. Recombinant proteins.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

Applications

Target

• RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 2932 Human
  • Entrez Gene: 2931 Human
  • Omim: 605004 Human
  • Omim: 606784 Human
  • SwissProt: P49840 Human
  • SwissProt: P49841 Human
  • Unigene: 466828 Human
  • Unigene: 445733 Human

• Alternative names
  Factor A antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibody

  GSK3 alpha antibodyGSK3 beta antibodyGSK3B antibody

  see all

Anti-GSK3 (alpha + beta) antibody images

• 

  Western blot - GSK3 (alpha + beta) antibody (ab82542)
  All lanes : Anti-GSK3 (alpha + beta) antibody (ab82542) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HEK-293T cell lysate
  Lane 3 : Jurkat cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 47 , 51 kDa
  Observed band size : 47,51 kDa