Anti-GSK3 (alpha + beta) antibody (ab82542)
- Product nameAnti-GSK3 (alpha + beta) antibodySee all GSK3 (alpha + beta) primary antibodies ...
- DescriptionRabbit polyclonal to GSK3 (alpha + beta)
- SpecificityRecognises both GSK3 alpha and GSK3 beta.
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 214-218 of Human GSK3 beta
- Positive controlJurkat cell lysate. Recombinant proteins.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
- Concentration information loading...
- PurityProtein A purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab82542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000. Predicted molecular weight: 47 , 51 kDa.|
- RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Cellular localizationCytoplasmic and Nuclear
- Factor A antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibody
- GSK3 alpha antibodyGSK3 beta antibodyGSK3B antibody
Anti-GSK3 (alpha + beta) antibody images
All lanes : Anti-GSK3 (alpha + beta) antibody (ab82542) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 47 , 51 kDa
Observed band size : 47,51 kDa
References for Anti-GSK3 (alpha + beta) antibody (ab82542)
This product has been referenced in:
- Banerji V et al. The intersection of genetic and chemical genomic screens identifies GSK-3a as a target in human acute myeloid leukemia. J Clin Invest 122:935-47 (2012). WB ; Human . Read more (PubMed: 22326953) »