Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)
Overview
- Product nameAnti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibodySee all GSK3 (alpha + beta) primary antibodies ...
- DescriptionRabbit polyclonal to GSK3 (alpha + beta) (phospho Y216 + Y279)
- SpecificityPhosphorylation site-specific antibody selective for the phosphorylated isoforms of glycogen synthase kinase enzyme, GSK 3 alpha, a 51 kDa protein containing a phosphate on tyrosine 279, and GSK 3 beta, a 47 kDa protein containing a phosphate on tyrosine 216.
- Tested applicationsWB, Dot Blot, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide derived from the regions of GSK 3 alpha + beta protein that contain tyrosine 216/279.
- Positive controlSerum starve 3T3-L1 cells for 3 hours, then stimulate with 100 nM insulin or 100 ng/mL IGF 1 for 5 min.
- General notes
Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from rabbit serum by epitope specific affinity chromatography. Any reactivity towards the non-tyrosine phosphorylated GSK 3 alpha + beta protein has been eliminated through a series of preabsorption steps.
- Primary antibody notes Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab4797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 51,47 kDa. Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 51kDa for GSK 3 alpha and 47 kDa for GSK 3 beta. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
- Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 2932 Human
- Entrez Gene: 2931 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 606496 Mouse
- Entrez Gene: 84027 Rat
- Entrez Gene: 50686 Rat
- Omim: 605004 Human
- Omim: 606784 Human
- SwissProt: P49840 Human
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- SwissProt: Q2NL51 Mouse
- SwissProt: P18265 Rat
- SwissProt: P18266 Rat
- Unigene: 466828 Human
- Unigene: 445733 Human
- Unigene: 36807 Rat
- Unigene: 10426 Rat
see all
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Alternative names
- C86142 antibodyDKFZp686D0638 antibodyFA antibody
- Factor A antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibodyGlycogen synthase kinase 3 beta antibodyGSK 3 alpha antibodyGSK 3 antibodyGSK 3 beta antibodyGSK 3 beta antibodyGSK 3A antibodyGSK 3B antibodyGSK3 alpha antibodyGSK3 beta antibodyGsk3a antibodyGSK3B antibodyKG3A antibodyKG3B antibody
see all
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody images
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Western blot - GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)
Predicted band size : 51,47 kDa
Rat PC12 cells were serum-starved or stimulated with 100 ng/mL NGF for 10 minutes. Cell lysates were generated and the NGF stimulated cells were treated with alkaline phosphatase for 30 minutes at 30ºC. The proteins were resolved by SDS-PAGE and transferred to PVDF membrane. Membranes were incubated with 1µg/mL pan GSK3alpha/beta and 0.5 µg/mL of ab4797. The signal was detected using a Goat F(ab’)2 anti-Rabbit IgG Alkaline Phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate using the WesternStarTM method (Tropix). The data show that the pan antibody recognizes both the alpha and beta form of GSK. In addition, there is strong basal phosphorylation of the tyrosine site, on alpha Y279 and beta site Y216. This is slightly enhanced by NGF treatment and fully dephosphorylated when extracts are treated with alkaline phosphatase.
References for Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody (ab4797)
This product has been referenced in:
- Jiang Y et al. Diabetes induces changes in ILK, PINCH and components of related pathways in the spinal cord of rats. Brain Res 1332:100-9 (2010). WB ; Rat . Read more (PubMed: 20347724) »
- Hartz AM et al. Estrogen receptor beta signaling through phosphatase and tensin homolog/phosphoinositide 3-kinase/Akt/glycogen synthase kinase 3 down-regulates blood-brain barrier breast cancer resistance protein. J Pharmacol Exp Ther 334:467-76 (2010). WB ; Rat . Read more (PubMed: 20460386) »
