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Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [0.T.57] (ab33021)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameAnti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [0.T.57]See all GSK3 (alpha + beta) primary antibodies ...
  • Description
    Mouse monoclonal [0.T.57] to GSK3 (alpha + beta) (phospho Y279 + Y216)
  • Tested applicationsWB, ICC more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide: C-KQLLHGEPNVSYpICSRY conjugated to Ovalbumin, corresponding to the catalytic domain (between subdomain VII and VIII), amino acids 203 - 219 of Fruit fly (Drosophila melanogaster) GSK3 (alpha + beta)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlJurkat lysate, NIH 3T3 lysate, A431 lysate and rat L6 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC
  • Application notesICC: Use at an assay dependent dilution.

    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 52 kDa. ab33021 detects GSK3 (alpha + beta) in RIPA lysates from human Jurkat cells, mouse 3T3, human A431 and rat L6 cell lysates. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab33021 (0.5ug/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceGlycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
      see all
    • Alternative names
        C86142 antibodyC86142 antibodyDKFZp686D0638 antibody
        FA antibodyFactor A antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen Synthase Kinase 3 Alpha and Beta antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase 3 beta antibodyGlycogen synthase kinase 3 beta antibodyGSK 3 alpha antibodyGSK 3 alpha antibodyGSK 3 antibodyGSK 3 beta antibodyGSK 3 beta antibodyGSK 3A antibodyGSK 3B antibodyGSK3 alpha antibodyGSK3 beta antibodyGsk3a antibodyGSK3B antibodyKG3A antibodyKG3A antibodyKG3B antibodyKG3B antibody
      see all

    References for Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [0.T.57] (ab33021)

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab33021 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab33021 Submit a Question for ab33021

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    Votes: Highest First

    Thank you very much for your reply. I' m interested in an anti-GSK3 that reacts in drosophila. Ideally the antibody is already tested for flow cytometry.

    Thank you for contacting us.

    Unfortunately, we don't have any other anti-GSK3 antibodies in our catalogue which is guaranteed to work in drosophila. In addition, the only 2 antibodies which are guaranteed to work in flow cytometry contain BSA. ...

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    could you please confirm, that the antibodies I asked for are supplied in a carrier free buffer.

    Thank you for contacting us.

    I can confirm that ab33021 contains no BSA only the following:
    Preservative: 0.05% Sodium Azide
    Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

    However, ab18893 conta...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"