Overview

  • Product nameAnti-GSK3 alpha (phospho S21) antibodySee all GSK3 alpha primary antibodies ...
  • Description
    Rabbit polyclonal to GSK3 alpha (phospho S21)
  • Tested applicationsICC/IF, ELISA, WB, IHC-P, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human GSK3a around the phosphorylation site of Serine 21.

Properties

Applications

Our Abpromise guarantee covers the use of ab28808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

Anti-GSK3 alpha (phospho S21) antibody images

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
  • All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution

    Lane 1 : Extracts from ovary cancer cells.
    Lane 2 : Extracts from ovary cancer cells. with synthesised non phosphopeptide at 1 µg/ml
    Lane 3 : Extracts from ovary cancer cells. with synthesised phosphopeptide at 1 µg/ml

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-GSK3 alpha (phospho S21) antibody (ab28808)

This product has been referenced in:
  • Yamaguchi N  et al. Dysfunctional ryanodine receptor and cardiac hypertrophy: role of signaling molecules. Am J Physiol Heart Circ Physiol 300:H2187-95 (2011). WB ; Mouse . Read more (PubMed: 21421818) »
  • Kapfhamer D  et al. Protein Phosphatase 2a and glycogen synthase kinase 3 signaling modulate prepulse inhibition of the acoustic startle response by altering cortical M-Type potassium channel activity. J Neurosci 30:8830-40 (2010). WB ; Mouse . Read more (PubMed: 20592205) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"