Anti-GSK3 alpha (phospho S21) antibody (ab28808)

Overview

• Product nameAnti-GSK3 alpha (phospho S21) antibodySee all GSK3 alpha primary antibodies ...
• Description
  Rabbit polyclonal to GSK3 alpha (phospho S21)
• Tested applicationsICC/IF, ELISA, WB, IHC-P, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  The antiserum was produced against synthesized phosphopeptide derived from human GSK3a around the phosphorylation site of Serine 21.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Cardiovascular
  • Heart
  • Apoptosis

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
  Contains 1 protein kinase domain.

Target information above from: UniProt accession P49840 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2931 Human
  • Entrez Gene: 2931 Human
  • Entrez Gene: 606496 Mouse
  • Entrez Gene: 50686 Rat
  • Omim: 606784 Human
  • SwissProt: P49840 Human
  • SwissProt: P49840 Human
  • SwissProt: Q2NL51 Mouse

  • SwissProt: P18265 Rat
  • Unigene: 466828 Human
  • Unigene: 294664 Mouse
  • Unigene: 36807 Rat

  see all

• Alternative names
  DKFZp686D0638 antibodyGlycogen synthase kinase 3 alpha antibodyGlycogen synthase kinase-3 alpha antibody

  GSK 3 alpha antibodyGSK 3 alpha antibodyGSK 3A antibodyGSK-3 alpha antibodyGSK3A antibodyGSK3A_HUMAN antibodyGSK3alpha antibodySerine/threonine protein kinase GSK3A antibody

  see all

Anti-GSK3 alpha (phospho S21) antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - GSK3 alpha (phospho S21) antibody (ab28808)
  Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
• 

  Western blot - GSK3 alpha (phospho S21) antibody (ab28808)
  All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution
  
  Lane 1 : Extracts from ovary cancer cells.
  Lane 2 : Extracts from ovary cancer cells. with synthesised non phosphopeptide at 1 µg/ml
  Lane 3 : Extracts from ovary cancer cells. with synthesised phosphopeptide at 1 µg/ml
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 50 kDa
  Observed band size : 50 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence-GSK3 alpha (phospho S21) antibody(ab28808)
  ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.