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Synthetic peptide: RARTSpSFAEPC, corresponding to amino acids 16-25 of Human GSK3 alpha.
Our Abpromise guarantee covers the use of ab9765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Rabbit polyclonal to phospho GSK alpha (Ser 21) at 1/1000 on 293T cell extract shows a band at 52 kD.
Cells were serum starved for 24 hours:
Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min
Lane 3 : pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min
Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada
ab9765 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"