Anti-GSK3 alpha (phospho S21) antibody (ab9765)
- Product nameAnti-GSK3 alpha (phospho S21) antibodySee all GSK3 alpha primary antibodies ...
- DescriptionRabbit polyclonal to GSK3 alpha (phospho S21)
- SpecificitySpecific for GSK 3 alpha isoform. Recognises a band at 52 kD.
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Synthetic peptide: RARTSpSFAEPC, corresponding to amino acids 16-25 of Human GSK3 alpha.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab9765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product has been assayed by ELISA against 0.1µg of the immunizing peptide.
A 1/1000 to 1/5000 dilution of the antibody is recommended for this assay.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
- FunctionImplicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
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Anti-GSK3 alpha (phospho S21) antibody images
Rabbit polyclonal to phospho GSK alpha (Ser 21) at 1/1000 on 293T cell extract shows a band at 52 kD.
Cells were serum starved for 24 hours:
Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min
Lane 3 : pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min
Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada
References for Anti-GSK3 alpha (phospho S21) antibody (ab9765)
ab9765 has not yet been referenced specifically in any publications.