Anti-GSK3 beta antibody [3D10] (ab93926)
Key features and details
- Mouse monoclonal [3D10] to GSK3 beta
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-GSK3 beta antibody [3D10]
See all GSK3 beta primary antibodies -
Description
Mouse monoclonal [3D10] to GSK3 beta -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Purified recombinant C-terminal fragment of Human GSK3 beta expressed in E. coli.
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Positive control
- WB: A549, K562, PC-12, NIH/3T3, and HEK293 cell lysates IHC-P: Lung cancer and breast cancer tissues ICC/IF: NIH/3T3 and U251 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D10 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
1/200 - 1/1000.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB | (5) |
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/4000 - 1/10000.
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Notes |
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ICC/IF
1/200 - 1/1000. |
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
IHC-P
1/4000 - 1/10000. |
Target
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Function
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
Tissue specificity
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 84027 Rat
- Omim: 605004 Human
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- SwissProt: P18266 Rat
- Unigene: 445733 Human
see all -
Alternative names
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
Images
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All lanes : Anti-GSK3 beta antibody [3D10] (ab93926) at 1/500 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : HEK293 cell lysate
Predicted band size: 47 kDa -
ab93926, at a 1/4000 dilution, staining GSK3 beta in NIH/3T3 cells (left) and U251 cells (right) by Immunofluorescence (green). DRAQ5 fluorescent DNA dye is blue and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
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ab93926, at a 1/4000 dilution, staining GSK3 beta in paraffin embedded Human lung cancer (left) and breast cancer (right) tissues by Immunohistochemistry. Detection: DAB staining.
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Overlay histogram showing HeLa cells stained with ab93926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93926, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (132)
ab93926 has been referenced in 132 publications.
- Lee HJ et al. Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke. Neural Regen Res 17:1556-1565 (2022). PubMed: 34916441
- Silva-Aguiar RP et al. Albumin Expands Albumin Reabsorption Capacity in Proximal Tubule Epithelial Cells through a Positive Feedback Loop between AKT and Megalin. Int J Mol Sci 23:N/A (2022). PubMed: 35055044
- Wang P et al. MicroRNA-1246 suppresses the metastasis of breast cancer cells by targeting the DYRK1A/PGRN axis to prevent the epithelial-mesenchymal transition. Mol Biol Rep 49:2711-2721 (2022). PubMed: 35059968
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Li W et al. miRNA-21 plays an important role in necrotizing enterocolitis. Arch Med Sci 18:406-412 (2022). PubMed: 35316914