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GSK3 beta (GSK3B) ELISA Kit (ab123454)
MS Catalog No. MS1007
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab123454? Please let us know so that we can cite the reference in this datasheet
ab123454 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Sandwich ELISA - GSK3 beta (GSK3B) ELISA Kit (ab123454)
Demonstration of assay specificity. Western blot using the GSK3B detector antibody (ab32391). HeLa cells treated with DMSO show a doublet band due to endogenous phosphorylation of the protein in this cell line. Wortmannin, a PI3-K inhibitor, dephosphorylates AKT and in-turn dephosphorylates GSK3B. Dephosphorylation removes the doublet band observed with vehicle treatment only.
Sandwich ELISA - GSK3 beta (GSK3B) ELISA Kit (ab123454)
Example standard curve. A serially diluted recombinant GSK3B protein (ab63193) in the working range of the assay
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