Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Proteins and Peptides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
GSK3 beta (GSK3B) ELISA Kit
Specificity
Species– Human, mouse and rat reactive. Others untested.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 8 | 4.6% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 3 | 15% |
Sample type
Cell Lysate
Assay type
Sandwich
Sensitivity
0.7 ng/ml
Range
1.56 ng/ml - 100 ng/ml
Cross reactivity
Reacts with
Mouse, Rat, Human
Product overview
ab123454 GSK3 beta (GSK3B) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of GSK3 beta protein in cell lysates. The assay employs an antibody specific for the Human GSK3 beta coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-GSK3 beta primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
If assay is to be performed on serum samples or samples with very low protein concentration, it is recommended to add serum or extraction buffer to the standard protein for accurate results.
Store all components at 4°C. This kit is stable for at least 6 months from receipt. A GSK3 beta standard protein is not included with the kit, but it is available from the Abcam catalogue (ab63193). Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 8206046 | 1 x 6ml |
| ab32391 - 10X GSK3 beta Detector Antibody | 8209547 | 1 x 0.7ml |
| 10X HRP Label | 8206026 | 1 x 1ml |
| 20X Buffer | 8209552 | 1 x 20ml |
| 2X Extraction Buffer | 8209533 | 1 x 15ml |
| ab93926 - GSK3 beta Microplate | 8209546 | 1 unit |
| TMB Development Solution | 8206045 | 1 x 6ml |
Function
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
Tissue specificity
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
Cellular localization
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
Target information above from: UniProt accessionP49841
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Glycogen synthase kinase-3 beta
- GSK-3 beta
- GSK3B
- GSK3B_HUMAN
Database links
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 84027 Rat
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- SwissProt: P18266 Rat
- Unigene: 445733 Human
- Unigene: 394930 Mouse
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent concentration.
GSK3 beta (GSK3B) ELISA Kit images:
Sandwich ELISA - GSK3 beta (GSK3B) ELISA Kit (ab123454)
Demonstration of assay specificity. Western blot using the GSK3B detector antibody (ab32391). HeLa cells treated with DMSO show a doublet band due to endogenous phosphorylation of the protein in this cell line. Wortmannin, a PI3-K inhibitor, dephosphorylates AKT and in-turn dephosphorylates GSK3B. Dephosphorylation removes the doublet band observed with vehicle treatment only.
Sandwich ELISA - GSK3 beta (GSK3B) ELISA Kit (ab123454)
Example standard curve. A serially diluted recombinant GSK3B protein (ab63193) in the working range of the assay
Protocols
References for GSK3 beta (GSK3B) ELISA Kit (ab123454)
ab123454 has not yet been referenced specifically in any publications.
Publishing research using ab123454? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"