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ab129730 is an In-Cell ELISA duplex assay panel that uses quantitative immunocytochemistry to measure GSK3B protein levels or post-translational modification (phospho S9) in cultured cells.
Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Glycogen synthase kinase 3 is a proline directed serine, threonine kinase originally identified due to its ability to phosphorylate and inactivate glycogen synthase and later found to be of key importance in signaling pathways, cell fate determination, energy metabolism, transcription regulation, neuronal development and body pattern formation. There are two isoforms of GSK-3 (alpha and beta) which show a high degree of homology within their catalytic domains.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
|Components||1 x 96 tests|
|1000X IRDye® Labeled Secondary Antibody Cocktail||1 x 24µl|
|100X Mouse Anti-GSK3 beta Total Primary Antibody||1 x 120µl|
|100X Rabbit Anti-GSK3 beta pSer9 Primary Antibody||1 x 120µl|
|10X Blocking Buffer||1 x 10ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|1X Janus Green Stain||1 x 11ml|
|30X Triton X-100 (10% solution)||1 x 1.5ml|
|400X Tween-20||1 x 2ml|
Our Abpromise guarantee covers the use of ab129730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab129730 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"