You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-GSK3 beta antibody [3D10] (ab93926)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-GSK3 beta antibody [3D10]
See all GSK3 beta products (24) ...

Description

Mouse monoclonal [3D10] to GSK3 beta

Tested applications

WB, ELISA, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Monkey

Immunogen

Purified recombinant C-terminal fragment of Human GSK3 beta expressed in E. coli.

Positive control

WB: A549, K562, PC-12, NIH/3T3, and HEK293 cell lysates IHC-P: Lung cancer and breast cancer tissues ICC/IF: NIH/3T3 and U251 cells

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Store at -20ºC.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

3D10

Isotype

IgG2a

Research areas

Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Notch Pathway

  • Western blot - GSK3 beta antibody [3D10] (ab93926)Western blot - GSK3 beta antibody [3D10] (ab93926) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 beta antibody [3D10] (ab93926)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 beta antibody [3D10] (ab93926) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - GSK3 beta antibody [3D10] (ab93926)Immunocytochemistry/ Immunofluorescence - GSK3 beta antibody [3D10] (ab93926) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab93926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/10000.
IHC-P: 1/4000 - 1/10000.
IHC-Fr: 1/4000 - 1/10000.
WB: 1/500 - 1/2000. Predicted molecular weight: 47 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

Tissue specificity

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.

Cellular localization

Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

Target information above from: UniProt accessionP49841 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Glycogen Synthase Kinase 3 Beta antibody
  • Glycogen synthase kinase-3 beta antibody
  • GSK 3 beta antibody
  • GSK-3 beta antibody
  • GSK3B antibody
  • GSK3B_HUMAN antibody
  • GSK3beta isoform antibody
see all

Database links

see all

Anti-GSK3 beta antibody [3D10] images:

  Western blot - GSK3 beta antibody [3D10] (ab93926)

Western blot - GSK3 beta antibody [3D10] (ab93926)

All lanes : Anti-GSK3 beta antibody [3D10] (ab93926) at 1/500 dilution

Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : HEK293 cell lysate


Predicted band size : 47 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 beta antibody [3D10] (ab93926)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 beta antibody [3D10] (ab93926)

ab93926, at a 1/4000 dilution, staining GSK3 beta in paraffin embedded Human lung cancer (left) and breast cancer (right) tissues by Immunohistochemistry. Detection: DAB staining.

  Immunocytochemistry/ Immunofluorescence - GSK3 beta antibody [3D10] (ab93926)

Immunocytochemistry/ Immunofluorescence - GSK3 beta antibody [3D10] (ab93926)

ab93926, at a 1/4000 dilution, staining GSK3 beta in NIH/3T3 cells (left) and U251 cells (right) by Immunofluorescence (green). DRAQ5 fluorescent DNA dye is blue and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

References for Anti-GSK3 beta antibody [3D10] (ab93926)

This product has been referenced in:

  • Dumont M  et al. Behavioral deficit, oxidative stress, and mitochondrial dysfunction precede tau pathology in P301S transgenic mice. FASEB J 25:4063-72 (2011). WB; Mouse.Read more (PubMed: 21825035) »

See 1 publication for this product

Publishing research using ab93926? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"