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Anti-GSK3 beta antibody [Y174] (ab32391)

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Overview

Product name

Anti-GSK3 beta antibody [Y174]
See all GSK3 beta products (24) ...

Description

Rabbit monoclonal [Y174] to GSK3 beta

Specificity

This antibody is specific for human GSK3 beta. It may also detect the splice isoform 2 based on sequence homology.

Tested applications

WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide (Human) corresponding to the C terminal peptide

Positive control

A431 cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y174

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Diabetes associated
Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Notch Pathway

  • Western blot - GSK3 beta antibody [Y174] (ab32391)Western blot - GSK3 beta antibody [Y174] (ab32391) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - GSK3 beta antibody [Y174] (ab32391)Immunohistochemistry (Paraffin-embedded sections) - GSK3 beta antibody [Y174] (ab32391) image (enlarge)

  • Western blot - GSK3 beta antibody [Y174] (ab32391)Western blot - GSK3 beta antibody [Y174] (ab32391) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

Tissue specificity

Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.

Cellular localization

Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

Target information above from: UniProt accessionP49841 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Glycogen Synthase Kinase 3 Beta antibody
  • Glycogen synthase kinase-3 beta antibody
  • GSK 3 beta antibody
  • GSK 3B antibody
  • GSK-3 beta antibody
  • GSK3B antibody
  • GSK3B protein antibody
  • GSK3B_HUMAN antibody
  • GSK3beta isoform antibody
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Database links

see all

Anti-GSK3 beta antibody [Y174] images:

  Western blot - GSK3 beta antibody [Y174] (ab32391)

Western blot - GSK3 beta antibody [Y174] (ab32391)

Anti-GSK3 beta antibody [Y174] (ab32391) at 1/10000 dilution + A431 cell lysate.

Predicted band size : 46 kDa
Observed band size : 46 kDa

  Immunohistochemistry (Paraffin-embedded sections) - GSK3 beta antibody [Y174] (ab32391)

Immunohistochemistry (Paraffin-embedded sections) - GSK3 beta antibody [Y174] (ab32391)

ab32391 at a 1:100 dilution staining GSK3 beta in human colon carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.

  Western blot - GSK3 beta antibody [Y174] (ab32391)

Western blot - GSK3 beta antibody [Y174] (ab32391)

All lanes : Anti-GSK3 beta antibody [Y174] (ab32391) at 1/2500 dilution

Lane 1 : Cell lysate prepared from MCF-7 cells
Lane 2 : Cell lysate prepared from SW480 cells

Lysates/proteins at 20 µg per lane.

Secondary
Donkey polyclonal IRDye 800CW at 1/15000 dilution

Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-GSK3 beta antibody [Y174] (ab32391)

This product has been referenced in:

  • Juhlin CC  et al. Loss of expression for the Wnt pathway components adenomatous polyposis coli and glycogen synthase kinase 3-beta in parathyroid carcinomas. Int J Oncol 34:481-92 (2009). IHC-P; Human.Read more (PubMed: 19148484) »

See 1 publication for this product

Publishing research using ab32391? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"