Anti-GSK3 beta antibody (ab227208)
Key features and details
- Rabbit polyclonal to GSK3 beta
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GSK3 beta antibody
See all GSK3 beta primary antibodies -
Description
Rabbit polyclonal to GSK3 beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Pig -
Immunogen
Recombinant fragment within Human GSK3 beta (internal sequence). The exact sequence is proprietary.
Database link: P49841 -
Positive control
- WB: HEK-293T and K562 whole cell extracts; Mouse brain lysate. IHC-P: H661 xenograft tissue; Rat brain tissue. ICC/IF: SK-N-SH cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
Target
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Function
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
Tissue specificity
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 790875 Cow
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Entrez Gene: 100126852 Pig
- Entrez Gene: 84027 Rat
- Entrez Gene: 100169938 Sheep
- Omim: 605004 Human
- SwissProt: P49841 Human
see all -
Alternative names
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
Images
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All lanes : Anti-GSK3 beta antibody (ab227208) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : GSK3 beta shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Anti-GSK3 beta antibody (ab227208) at 1/3000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Anti-GSK3 beta antibody (ab227208) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Paraffin-embedded H661 (human) xenograft tissue stained for GSK3 beta using ab227208 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for GSK3 beta using ab227208 at 1/500 dilution in immunohistochemical analysis.
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4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for GSK3 beta (green) using ab227208 at 1/400 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab227208 has been referenced in 4 publications.
- Cai J et al. SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35419615
- Fu C et al. CircTET1 Inhibits Retinoblastoma Progression via Targeting miR-492 and miR-494-3p through Wnt/ß-catenin Signaling Pathway. Curr Eye Res N/A:1-10 (2021). PubMed: 33108919
- Wang H et al. MicroRNA-15a-5p inhibits endometrial carcinoma proliferation, invasion and migration via downregulation of VEGFA and inhibition of the Wnt/ß-catenin signaling pathway. Oncol Lett 21:310 (2021). PubMed: 33732386
- Kim EH et al. Effect of Klotho protein during porcine oocyte maturation via Wnt signaling. Aging (Albany NY) 12:23808-23821 (2020). PubMed: 33176278