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GST Activity Assay Kit (Fluorometric) (ab65325)

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Overview

  • Product nameGST Activity Assay Kit (Fluorometric)See all GST kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    < 0.5 mU/well
  • Assay time
    2h 00m
  • Product overview

    Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when reacts with glutathione. GST catalyzes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
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  • Notes

    Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta.
  • Cellular localizationCytoplasmic
    • Alternative names
        Glutathione S transferase A1Glutathione S transferase A2GST class alpha 1
        GST class alpha 2GST epsilonGST gammaGSTA1GSTA2GTH1GTH2HA subunit 1HA subunit 2
      see all

    Applications

    Our Abpromise guarantee covers the use of ab65325 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    GST Activity Assay Kit (Fluorometric) images

    • Functional Studies - GST Activity Assay Kit (Fluorometric) (ab65325)
      Functional Studies - GST Activity Assay Kit (Fluorometric) (ab65325)
      Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.

    Protocols

    References for GST Activity Assay Kit (Fluorometric) (ab65325)

    ab65325 has not yet been referenced specifically in any publications.

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